Nat Prod Sci.  2019 Mar;25(1):76-80. 10.20307/nps.2019.25.1.76.

Nitric Oxide Inhibitory Constituents from the Fruits of Amomum tsao-ko

• Kim JG
• Le TP
• Hong HR
• Han JS
• Ko JH
• Lee SH
• Lee MK
• Hwang BY

• KMID: 2443108
• DOI: http://doi.org/10.20307/nps.2019.25.1.76

Abstract

Bioactivity-guided fractionation of MeOH extract of the dried fruits of Amomum tsao-ko led to isolation of nine compounds (1 - 9). Their structures were elucidated by spectroscopic methods including extensive 1D and 2D-NMR, as alpinetin (1), naringenin-5-O-methyl ether (2), naringenin (3), hesperetin (4), 2"²,4"²,6"²-trihydroxy-4-methoxy chalcone (5), tsaokoin (6), boesenbergin B (7), 4-hydroxyboesenbergin B (8), and tsaokoarylone (9). Of these, compound 8 was isolated from a natural source for the first time, which was previously reported as a synthetic product. The isolated compounds (1 - 9) were tested for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Among them, three chalcone derivatives (compounds 5, 7, and 8) and a diarylheptanoid (compound 9) exhibited significant inhibitory activity on the NO production with ICâ‚…â‚€ values ranging from 10.9 to 22.5 µM.

Keyword

Amomum tsao-ko; Zingiberaceae; Nitric oxide; Anti-inflammatory activity

MeSH Terms

Amomum*
Chalcone
Ether
Fruit*
Macrophages
Nitric Oxide*
Zingiberaceae
Chalcone
Ether
Nitric Oxide

Figure

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  Fig. 1 The structures of 1 – 9 isolated from A. tsao-ko.

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  Fig. 2 Key HMBC correlations for compound 8.

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