Allergy Asthma Immunol Res.  2019 May;11(3):422-432. 10.4168/aair.2019.11.3.422.

Validation of PROTIA™ Allergy-Q 64 Atopy® as a Specific IgE Measurement Assay for 10 Major Allergen Components

• Kim SR
• Park KH
• Lee JH
• Kim BJ
• Hwang JH
• Lim KJ
• Park JW

• KMID: 2442052
• DOI: http://doi.org/10.4168/aair.2019.11.3.422

Abstract

PURPOSE
Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIAâ„¢ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components.
METHODS
The sIgE detection by PROTIAâ„¢ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIAâ„¢ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays.
RESULTS
Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743).
CONCLUSIONS
PROTIAâ„¢ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

Keyword

Immunoassay; immunoglobulin E; allergens

MeSH Terms

Allergens
Caseins
Dermatophagoides farinae
Dermatophagoides pteronyssinus
Diagnosis
Gliadin
Humans
Immunoassay
Immunoglobulin E*
Immunoglobulins
Pyroglyphidae
Allergens
Caseins
Gliadin
Immunoglobulin E
Immunoglobulins

Figure

• 

  Figure Intra-class comparison of ImmunoCAP® with PROTIATM Allergy-Q 64 Atopy®. Measured sIgEs were classified as class 0: below 0.35 kUA/L, class 1: 0.35-0.7 kUA/L, class 2: 0.7-3.5 kUA/L, class 3: 3.5-17.5 kUA/L, class 4: 17.5-50 kUA/L, class 5: 50-100 kUA/L and class 6: above 100 kUA/L. Statistical values for intra-class comparison are shown in Table 4. Der p, Dermatophagoides pteronyssinus; Der f, Dermatophagoides farina.

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