Allergy Asthma Immunol Res.  2019 May;11(3):422-432. 10.4168/aair.2019.11.3.422.

Validation of PROTIA™ Allergy-Q 64 Atopy® as a Specific IgE Measurement Assay for 10 Major Allergen Components

Affiliations
  • 1Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. PARKJW@yuhs.ac
  • 2Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
  • 3Proteometech Inc., Seoul, Korea.

Abstract

PURPOSE
Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIAâ„¢ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components.
METHODS
The sIgE detection by PROTIAâ„¢ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIAâ„¢ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays.
RESULTS
Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743).
CONCLUSIONS
PROTIAâ„¢ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

Keyword

Immunoassay; immunoglobulin E; allergens

MeSH Terms

Allergens
Caseins
Dermatophagoides farinae
Dermatophagoides pteronyssinus
Diagnosis
Gliadin
Humans
Immunoassay
Immunoglobulin E*
Immunoglobulins
Pyroglyphidae
Allergens
Caseins
Gliadin
Immunoglobulin E
Immunoglobulins
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