Korean J Pediatr Infect Dis.  1997 Nov;4(2):218-224. 10.14776/kjpid.1997.4.2.218.

Oropharyngeal Carriage and Antimicrobial Resistance of S. pneumoniae in Children of Seoul

• Kim YK
• Lee CK

• KMID: 2440335
• DOI: http://doi.org/10.14776/kjpid.1997.4.2.218

Abstract

PURPOSE
The antimicrobial resistance of S. pneumoniae has encountered with increasing frequency from around the world. In our country, penicillin resistant strains of S. penumococci are rapidly increasing. It has been blown that colonized pneumococci in upper respiratory tract cause sinisitis, otitis media, meningitis and pneumonia. We tried to reveal the colonization rate of pneumonocci in upper respiratory tract, their antimicrobial resistance and DNA fingerprinting pattern in normal children.
METHODS
We got specimens from 117 children of day-care center in Seoul through oropharyngeal swab. After incubation on BAP, optochin test and slide latex agglutination test were used for identification. Antimicobial susceptibility test to penicillin, vancomycin, erythromycin and TMP-SMZ was done with disk diffusion method. Penicillin MIC was gotten through the broth microdilution method. Genotyping of 45 pneumococci was done by rep-PCR using REP1R-Dt and REP2-Dt primer.
RESULTS
The carriage rate of pneumococci in the day-care center children was 38%(45/117). The resistance of penicillin, erhthromycin, TMP/SMZ, vancomycin by the disk diffusion method are 89%, 91%, 64% and 0%, respectively. 64% of the isolates showed multiple resistance. 7 types of DNA fingerprinting were gotten and 78% of isolates belonged to three types.
CONCLUSION
We found that the antimicrobial resistance of children attending the day-care center in Seoul was much higher than expected. We assumed that this might be due to their easy and frequent exposure to antimicrobial agents and crowded day-care center environment.

Keyword

Pneumococci; Carriage; Antimicrobial resistance

MeSH Terms

Anti-Infective Agents
Child*
Colon
Diffusion
DNA Fingerprinting
Erythromycin
Humans
Latex Fixation Tests
Meningitis
Methods
Otitis Media
Penicillins
Pneumonia*
Respiratory System
Seoul*
Vancomycin
Anti-Infective Agents
Erythromycin
Penicillins
Vancomycin