Korean J Pediatr Infect Dis.  1999 May;6(1):123-130. 10.14776/kjpid.1999.6.1.123.

Enterovirus Sequencing Analysis of 5

• Chung MA
• Lou CW
• Kim DS
• Yun JD
• Kim KS
• Lee YS

• KMID: 2440281
• DOI: http://doi.org/10.14776/kjpid.1999.6.1.123

Abstract

PURPOSE
Meningitis is an inflammation of meninges by various kinds of organisms. Almost 85% of aseptic meningitis is caused by Enterovirus. This study was done to detect the causative virus of those with aseptic meningitis through sequencing the 5"²-noncoding region to compare prototype and homology.
METHODS
RNA was extracted from Coxsackie Bl, Echovirus 3, 7, 9, 30. DNA was synthesized by RT-PCR and we compared homology with prototype from WHO by direct sequencing.
RESULTS
1) PCR products from these viruses showed same bands of 155 bp and 440 Up on gel electrophoresis. 2) Coxsackievirus and Echovirus 11 prototype sequences were compared, which showed 12 bp changes with 92.1%. 3) Coxsackievirus B1 from a patient showed 94.1% homology when compared with prototype. 4) Echovirus 3 showed 92.8%, echovirus 7 92.8%, echovirus 30 82.9% homology.
CONCLUSION
5"²-NCR of enterovirus has high homology which was good for use of diagnosis and more long sequencing requires for typing of viruses.

Keyword

Aseptic menigitis; Enterovirus

MeSH Terms

Diagnosis
DNA
Electrophoresis
Enterovirus B, Human
Enterovirus*
Humans
Inflammation
Meninges
Meningitis
Meningitis, Aseptic
Polymerase Chain Reaction
RNA
DNA
RNA