Korean J Pediatr Infect Dis.  2004 Nov;11(2):153-157. 10.14776/kjpid.2004.11.2.153.

Prevalence of Transfusion Transmitted Virus-Like Mini Virus in Children

• Chung JY
• Han TH

• KMID: 2440061
• DOI: http://doi.org/10.14776/kjpid.2004.11.2.153

Abstract

PURPOSE
Ttransfusion transmitted virus(TTV) is a circular DNA and consists of diverse genotypes and variants. The pathogenecity of TTV is still unclear. Recently another circular single stranded DNA virus, distantly related to TTV was isolated from the sera of blood donors, designated as Transfusion transmitted virus like minivirus(TLMV). TTV and TLMV show greater sequence divergence from each other than between genotypes of TTV. We planned to know the prevalence of TLMV in children.
METHODS
TLMV DNA was detected by PCR primers from noncoding region of the genome in 88 children without hepatitis, aged 0~15 years. PCR products derived from 10 children were directly sequenced and phylogenetic analysis was undertaken.
RESULTS
TLMV DNA was detected in 49% of 88 children without hepatitis. The prevalence of TLMV varied with age : < 1 y, 16%(4/25); 1~3 y, 62%(18/29); 4~6 y, 43%(7/16); 7~9 y. 16%(1/6); 10~15 y, 66%(8/12). Mixed infection with TTV was confirmed in 22% of 88 children. Pyhlogenetic analysis of 10 TLMV sequences showed much heterogeneity compared to sequences of GenBank.
CONCLUSION
TLMV prevalence in children was 49% in Korean children. Our TLMV sequence did not cluster in any sequence of TLMV in the GenBank.

Keyword

Transfusion transmitted virus-like mini virus(TLMV); Children

MeSH Terms

Blood Donors
Child*
Coinfection
Databases, Nucleic Acid
DNA
DNA, Circular
DNA, Single-Stranded
Genome
Genotype
Hepatitis
Humans
Polymerase Chain Reaction
Population Characteristics
Prevalence*
Torque teno virus
DNA
DNA, Circular
DNA, Single-Stranded