Korean J Pediatr Gastroenterol Nutr.  2005 Sep;8(2):202-212.

Prevalence and Genotypes of Transfusion-Transmitted Virus in Children with Hepatitis and Normal Control

• Chung JY
• Han TH
• Hwang ES
• Ko JS
• Seo JK

Abstract

PURPOSE: Transfusion transmitted virus (TTV) is a newly discovered virus and to date the contribution of TTV to liver disease remains unclear. Little is known about the frequency of TTV infection in children in Korea. The purpose of this study was to investigate the prevalence and genotypic distribution of TTV carried by healthy children and patients with hepatitis in Korea.
METHODS
Eighty eight of healthy children and three groups of patients with hepatitis-14 patients with chronic hepatitis B, 12 patients with chronic hepatitis C and 25 patients with hepatitis of unknown etiology-were tested. TTV DNA was detected by semi-nested PCR using primer sets generated from N-22 region and from 5' noncoding region (NCR) of the viral genome. PCR products derived from 8 patients with hepatitis and from 11 healthy children were sequenced and a phylogenetic tree was constructed.
RESULTS
TTV was found by PCR with N22 primer in 11.3% of healthy children, 28.5% of children with hepatitis B, 25% of children with hepatitis C, 24% of children with hepatitis of unknown etiology. TTV DNA was found by PCR with 5'NCR primer in 32.9% of healthy children, 71.4% of patients with chronic hepatitis B, in 50% of patients with hepatitis C and in 48% of patients with hepatitis of unknown etiology. TLMV DNA was found in 48.9% of healthy children, 21.4% of patients with hepatitis B, 16.6% of patients with hepatitis C, 40% of patients with hepatitis of unknown etiology. Among the sequenced isolates, 10(52%) belonged to genotype 1 (G1) and others belonged to genotype 2 (G2) or genotype 3 (G3). Among the G1 sequences, 7 were grouped as G1a.
CONCLUSION
TTV infection was common in healthy children and in patients with hepatitis. But, the prevalence of TTV DNA by 5'NCR primer was relatively high in patients with hepatitis B and there may be some association between TTV and hepatitis B virus infection. G1 was the major genotype of the studied population.

Keyword

Transfusion-transmitted virus; Hepatitis; Children; Genotype

MeSH Terms

Child*
DNA
Genome, Viral
Genotype*
Hepatitis B
Hepatitis B virus
Hepatitis B, Chronic
Hepatitis C
Hepatitis C, Chronic
Hepatitis*
Humans
Korea
Liver Diseases
Polymerase Chain Reaction
Prevalence*
Torque teno virus*
DNA