Nat Prod Sci.  2018 Dec;24(4):288-292. 10.20307/nps.2018.24.4.288.

Efficient Isolation of Dihydrophaseic acid 3′-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography

Affiliations
  • 1College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon14662, Korea. kdyoon@catholic.ac.kr

Abstract

High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3"²-O-β-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

Keyword

Nelumbo nucifera; Dihydrophaseic acid 3′-O-β-D-glucopyranoside; High-performance countercurrent chromatography; Isolation efficiency

MeSH Terms

Chromatography
Chromatography, Liquid*
Countercurrent Distribution*
Methods
Nelumbo*
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