Korean J Parasitol.
2018 Oct;56(5):437-446. 10.3347/kjp.2018.56.5.437.
Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
- Affiliations
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- 1Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea. hongsj@cau.ac.kr
- 2Department of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. younhj@snu.ac.kr
- 3Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Gwangju 61469, Korea.
- 4Department of Veterinary Parasitology, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
- 5Department of Parasitology, College of Medicine, Catholic University, Seoul 06591, Korea.
- 6Division of Planning and Management, Nakdong-gang National Institute of Biological Resources, Sangju 37242, Korea.
- 7Department of Veterinary Pathology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
- 8Department of Veterinary Parasitology, Kyungpook National University College of Veterinary Sciences, Daegu 41566, Korea.
- 9Parasitic and Insect Disease Laboratory, Bacterial and Parasitic Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- 10VexPert Co., Suwon 16614, Korea.
- 11KoreaBioGroup Co., Incheon 22013, Korea.
- 12Department of Parasitology and Tropical Medicine and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 22333, Korea.
- 13State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, P. R. China.
- 14Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P. R. China.
- KMID: 2426748
- DOI: http://doi.org/10.3347/kjp.2018.56.5.437
Abstract
- To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
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