J Korean Pain Soc.
1998 Oct;11(2):182-193.
Increased CNTF Expression in the Reactive Astrocyte Following Spinal Cord Injury in Rats
- Affiliations
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- 1Department of Anesthesiology, College of Medicine, The Catholic University of Korea, Korea.
- 2Department of Anatomy, College of Medicine, The Catholic University of Korea, Korea.
Abstract
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BACKGROUND: Ciliary neurotrophic factor (CNTF), identified as a survival factor for developing peripheral neurons
is upregulated by reactive astmcytes in the traumatized tissue and in areas of terminal degeneratian after a brain lesion.
But in the spinal cord, CNTF is expressed in the non-astrocytic phenotypic, maybe oligodendrocytes. The present study was
undertaken to determine the upregulation of CNTF expression in reactive astrocytes following spinal cord lesion in the rat.
METHODS
Unilateral incision of the dorsal funiculus at the thoracic level was performed and rats were sacrificed on days 3, 7, 14 and 28 postlesion. Western blot analysis, immunocytochemical analysis and double immunofluorescence for CNTF and glial fibrillary acidic protein (GFAP) were performed after spinal cord lesion.
RESULTS
A major band with 24 kDa and additianal band of higher molecular weight form were detectable, and the intensity of the 24 kDa immunoreactive band increased up to 14 days postlesion and decreased toward huninectomized control values. CNTF immunoreactivity was markedly upregulated in the injured dorsal funiculus and adjacent gray matter. The time course of CNTF expression is coincident with the appearance of reactive astrocytes in the injured spinal cord. Moreover, double immunofluoamcence for CNTF and glial fibrillary acidic protein (GFAP) revealed that CNTF immunoreactivity was in GFAP immunoreactive astrocytes.
CONCLUSIONS
These results show that CNTF upregulation occurred in reactive astrocytes following spinal cord lesion, and suggest a role for CNTF in the regulation of astrocytic responses after spinal cord injury.
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