J Korean Med Sci.  2018 Aug;33(33):e224. 10.3346/jkms.2018.33.e224.

Marker Pen Device with Concentration Gradient Nib for Antibiotic Susceptibility Testing

• Jung YG
• Yun YR
• Song SH
• Park W

• KMID: 2418428
• DOI: http://doi.org/10.3346/jkms.2018.33.e224

Abstract

BACKGROUND
Pen-based devices have emerged as useful tools for measuring pH and glucose, and for fabricating microchannels and microarrays. Pen-based devices take advantage of flexible patterning, inexpensive costs, and small volumes, thereby saving time and increasing efficiency. We have developed a gradient nib marker pen device that generated simultaneously different antibiotic concentrations in bacteria antibiotic susceptibility testing (AST).
METHODS
The device can deposit on the target surface with the antibiotic gradient. The designed polyester fiber nibs are a highly uniform porosity with unidirectional orientation and produce a visible gradient pattern.
RESULTS
We have demonstrated and quantitatively analyzed bacterial growth after antibiotic marking. The antibiotic marking produces an inhibition zone of bacterial growth. The inhibition zones of bacterial growth are captured and converted to 8-bit grayscale images, and then quantified by gray values using the Image J program. A profile of the inhibition zone showed different gray values in response to bacterial viability.
CONCLUSION
The gradient nib marker pen device can be used to determine the quantitative antibiotic concentration based on the relationship between gray values and bacterial density conveniently without requiring a series of dilution tubes, including nutrient medium, and diversely diluted antibiotics.

Keyword

Marker Pen; Concentration Gradient; Nib, Antibiotic; Susceptibility

MeSH Terms

Anti-Bacterial Agents
Bacteria
Clothing
Glucose
Hydrogen-Ion Concentration
Microbial Viability
Polyesters
Porosity
Anti-Bacterial Agents
Glucose
Polyesters