J Cancer Prev.  2018 Jun;23(2):82-86. 10.15430/JCP.2018.23.2.82.

Characterization of JAK2 V617F (1849 G > T) Mutation in Cervical Cancer Related to Human Papillomavirus and Sexually Transmitted Infections

• Abdolmaleki M
• Sohrabi A

• KMID: 2417371
• DOI: http://doi.org/10.15430/JCP.2018.23.2.82

Abstract

BACKGROUND
Human papillomavirus (HPV) genotypes that infect the genital tract play a main etiologic role in cervical cancer progression. Other environmental factors, such as sexually transmitted diseases and the host genetic pattern, contribute to infection persistence of the uterus and cervical epithelium in sustaining their malignancy. The Janus kinase 2 is a non-receptor tyrosine kinase in cell signaling process of tumor genesis. In the present study, JAK2 V167F mutation was distinguished in women with sexually transmitted infections, such as Herpes simplex virus 2, Chlamydia trachomatis and Mycoplasma genitalium and cervical cancer.
METHODS
This case-control survey was performed on 195 liquid based cytology of women specimens. Fifty, 98, and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. Single nucleotide polymorphism analysis, sexually transmitted infections detection and HPV genotyping were carried out using approved PCR- RFLP, in-house multiplex TaqMan Real Time PCR and the reverse dot blot hybridization assay.
RESULTS
HPVs 6, 16, 18, 11, 31, and 51 were the most common genotypes. The prevalence rate of multiple HPV genotypes was 46.0% to 10.1%. Analysis of JAK2 V617F (1849 G > T) showed that prevalence of mutation was GG (65.1%), GA (34.9%), and TT (0%), respectively. There were no statistically significant differences between this mutation and variables of population survey (P ≥ 0.05).
CONCLUSIONS
The molecular epidemiology study on the genetic polymorphisms, i.e., JAK2 V617F and other single nucleotide polymorphisms as a diagnostic tool is necessary for cancer screening and prophylactic programs.

Keyword

Human papillomavirus; Sexually transmitted infections; Janus kinase 2; Polymorphism; Cervical cancer; Iran

MeSH Terms

Case-Control Studies
Chlamydia trachomatis
Early Detection of Cancer
Epithelium
Female
Genotype
Herpesvirus 2, Human
Humans*
Iran
Janus Kinase 2
Molecular Epidemiology
Mycoplasma genitalium
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Prevalence
Protein-Tyrosine Kinases
Real-Time Polymerase Chain Reaction
Sexually Transmitted Diseases*
Uterine Cervical Neoplasms*
Uterus
Janus Kinase 2
Protein-Tyrosine Kinases