Blood Res.  2017 Jun;52(2):112-118. 10.5045/br.2017.52.2.112.

Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia

• Dasgupta S
• Ray UK
• Mitra AG
• Bhattacharyya DM
• Mukhopadhyay A
• Das P
• Gangopadhyay S
• Roy S
• Mukhopadhyay S

• KMID: 2413328
• DOI: http://doi.org/10.5045/br.2017.52.2.112

Abstract

BACKGROUND
Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitoring of CML. Previously, many technologies, most of which are laborious and time consuming, have been developed to detect BCR-ABL chimeric gene or chromosome.
METHODS
A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion proteins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated.
RESULTS
From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantitative real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR.
CONCLUSION
BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.

Keyword

Chronic myeloid leukemia; BCR-ABL1; Philadelphia chromosome; Flow cytometry

MeSH Terms

Arm
Cytogenetics
Diagnosis
Flow Cytometry*
Fusion Proteins, bcr-abl
Humans
Karyotyping
Leukemia, Myelogenous, Chronic, BCR-ABL Positive*
Methods*
Philadelphia Chromosome
Real-Time Polymerase Chain Reaction
Fusion Proteins, bcr-abl

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