J Vet Sci.  2016 Sep;17(3):427-429. 10.4142/jvs.2016.17.3.427.

Mycobacterium bovis infection in a wild sow (Sus scrofa): the first case in Korea

• Ku BK
• Jeon BY
• Kim JM
• Jang YB
• Jang Y
• Yu SY
• Kim J
• Moon OK
• Jung SC
• Lee MK
• Jeong TN

• KMID: 2413145
• DOI: http://doi.org/10.4142/jvs.2016.17.3.427

Abstract

Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea.

Keyword

Korea; Mycobacterium bovis; wild sow

MeSH Terms

Animals
Female
Mycobacterium bovis/*isolation & purification
Republic of Korea
Swine
Swine Diseases/*diagnosis/microbiology/pathology
Tuberculosis/diagnosis/microbiology/pathology/*veterinary

Figure

• 

  Fig. 1 Visible lesions and Ziehl-Neelsen staining of the lungs of wild sow. (A) Visible lesions in the nodules of the lung from a wild sow showing well-demarcated yellow caseous nodules (arrowheads) at the cut surface. (B) Ziehl–Neelsen staining of a lung section nodule shows acid-fast bacilli (arrows) in a granuloma. Scale bar = 10 µm.

• 

  Fig. 2 Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.

Reference

1. Barasona JA, Latham MC, Acevedo P, Armenteros JA, Latham ADM, Gortazar C, Carro F, Soriguer RC, Vicente J. Spatiotemporal interactions between wild boar and cattle: implications for cross-species disease transmission. Vet Res. 2014; 45:122.

2. Fitzgerald SD, Kaneene JB. Wildlife reservoirs of bovine tuberculosis worldwide: hosts, pathology, surveillance, and control. Vet Pathol. 2013; 50:488–499.

3. Je S, Ku BK, Jeon BY, Kim JM, Jung SC, Cho SN. Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing. Vet Microbiol. 2015; 176:274–281.

4. Jeon BY, Je S, Park J, Kim Y, Lee EG, Lee H, Seo S, Cho SN. Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea. J Vet Sci. 2008; 9:145–153.

5. Kim Y, Choi Y, Jeon BY, Jin H, Cho SN, Lee H. A simple and efficient multiplex PCR assay for the identification of Mycobacterium genus and Mycobacterium tuberculosis complex to the species level. Yonsei Med J. 2013; 54:1220–1226.

6. Naranjo V, Gortazar C, Vicente J, de la Fuente J. Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex. Vet Microbiol. 2008; 127:1–9.

7. Palmer MV. Mycobacterium bovis: characteristics of wildlife reservoir hosts. Transbound Emerg Dis. 2013; 60:Suppl 1. 1–13.

8. Pesciaroli M, Alvarez J, Boniotti MB, Cagiola M, Di Marco V, Marianelli C, Pacciarini M, Pasquali P. Tuberculosis in domestic animal species. Res Vet Sci. 2014; 97:Suppl. S78–S85.

9. Santos N, Correia-Neves M, Ghebremichael S, Källenius G, Svenson SB, Almeida V. Epidemiology of Mycobacterium bovis infection in wild boar (Sus scrofa) from Portugal. J Wildl Dis. 2009; 45:1048–1061.