Gut Liver.
2016 Jul;10(4):624-631. 10.5009/gnl15120.
Ascitic Fluid Calprotectin and Serum Procalcitonin as Accurate Diagnostic Markers for Spontaneous Bacterial Peritonitis
- Affiliations
-
- 1Department of Tropical Medicine, Mansoura University, Mansoura, Egypt. Mousa_medic@yahoo.com
- 2Department of Clinical Pathology, Mansoura University, Mansoura, Egypt.
- 3Department of Endemic Diseases and Gastroenterology, Aswan University, Aswan, Egypt.
- 4Department of Internal Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
- 5Department of Medical Microbiology and Immunology, Mansoura University, Mansoura, Egypt.
- KMID: 2412053
- DOI: http://doi.org/10.5009/gnl15120
Abstract
- BACKGROUND/AIMS
The diagnosis of spontaneous bacterial peritonitis (SBP) is based on a polymorphonuclear leukocytes (PMNs) exceeding 250/μL in ascitic fluid. The aim of the study was to evaluate serum procalcitonin and ascitic fluid calprotectin as accurate diagnostic markers for detecting SBP.
METHODS
Seventy-nine patients with cirrhotic ascites were included. They were divided into a SBP group, including 52 patients, and a non-SBP group of 27 patients. Serum procalcitonin, ascitic calprotectin, and serum and ascitic levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured using an enzyme-linked immunosorbent assay.
RESULTS
Serum procalcitonin and ascitic calprotectin were significantly higher in SBP patients than in non-SBP patients. Significant increases in both serum and ascitic levels of TNF-α and IL-6 were observed in SBP patients versus non-SBP patients. At a cutoff value of 0.94 ng/mL, serum procalcitonin had 94.3% sensitivity and 91.8% specificity for detecting SBP. In addition, at a cutoff value of 445 ng/mL, ascitic calprotectin had 95.4% sensitivity and 85.2% specificity for detecting SBP. Both were positively correlated with ascitic fluid proteins, PMN count, TNF-α, and IL-6.
CONCLUSIONS
According to our findings, determination of serum procalcitonin levels and ascitic calprotectin appears to provide satisfactory diagnostic markers for the diagnosis of SBP.
Keyword
MeSH Terms
-
Aged
Ascitic Fluid/*chemistry
Bacterial Infections/*diagnosis/microbiology
Biomarkers/analysis
Calcitonin/*blood
Enzyme-Linked Immunosorbent Assay
Female
Humans
Interleukin-6/analysis
Leukocyte L1 Antigen Complex/*analysis
Liver Cirrhosis/blood/*metabolism/microbiology
Male
Middle Aged
Peritonitis/*diagnosis/microbiology
Reference Values
Sensitivity and Specificity
Tumor Necrosis Factor-alpha/analysis
Biomarkers
Interleukin-6
Leukocyte L1 Antigen Complex
Tumor Necrosis Factor-alpha
Calcitonin
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- A Prospective, Comparative Study of Two Methods of Ascitic Fluid Culture to Diagnose Spontaneous Bacterial Peritonitis.
- Procalcitonin and C-Reactive Protein in the Diagnosis and Prediction of Spontaneous Bacterial Peritonitis Associated With Chronic Severe Hepatitis B
- Tumor Necrosis Factor-a and Interleukin- in Ascitic Fluid and Plasma in Spontaneous Bacterial Peritonitis
- Comparison of Ascitic Fluid Culture Methods for Diagnosing Spontaneous Bacterial Peritonitis
- Clinical Significance of CA 125 in Patients with Tuberculous Peritonitis
