Tissue Eng Regen Med.
2017 Dec;14(6):803-814. 10.1007/s13770-017-0076-8.
Calcium Microcrystal Formation in Recurrent Herniation Patients After Autologous Disc Cell Transplantation
- Affiliations
-
- 1Translational Centre of Regenerative Medicine TRM, University of Leipzig, Philipp-Rosenthal-Straße 55, 04103 Leipzig, Germany. Stefan.Schwan@imws.fraunhofer.de
- 2Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Huelse-Str. 1, 06120 Halle (Saale), Germany.
- 3Department of Chemical and Biomolecular Engineering, University of Tennessee, 1512 Middle Drive, Knoxville, TN 37996, USA.
- 4Department of Trauma Surgery, Friedrich-Schiller-University, Am Klinikum 1, 07747 Jena, Germany.
- 5Department of Trauma Surgery, BG-Klinikum Bergmannstrost Halle, Merseburger Straße 165, 06112 Halle (Saale), Germany.
- 6Department of Diagnostic Imaging and Interventional Radiology, BG-Klinikum Bergmannstrost Halle, Merseburger Straße 165, 06112 Halle (Saale), Germany.
- 7Department of Neurosurgery, BG-Klinikum Bergmannstrost Halle, Merseburger Straße 165, 06112 Halle (Saale), Germany.
- 8Department of Neurosurgery, Helsinki University Central Hospital, University of Helsinki, Topeliuksenkatu 5, 00029 Helsinki, Finland.
- KMID: 2411873
- DOI: http://doi.org/10.1007/s13770-017-0076-8
Abstract
- Autologous disc cell transplantation (ADCT) is a cell-based therapy aiming to initiate regeneration of intervertebral disc (IVD) tissue, but little is known about potential risks. This study aims to investigate the presence of structural phenomena accompanying the transformation process after ADCT treatment in IVD disease. Structural phenomena of ADCT-treated patients (Group 1, n = 10) with recurrent disc herniation were compared to conventionally-treated patients with recurrent herniation (Group 2, n = 10) and patients with a first-time herniation (Group 3, n = 10). For ethical reasons, a control group of ADCT patients who did not have a recurrent disc herniation was not possible. Tissue samples were obtained via micro-sequestrectomy after disc herniation and analyzed by micro-computed tomography, scanning electron microscopy, energy dispersive spectroscopy, and histology in terms of calcification zones, tissue structure, cell density, cell morphology, and elemental composition. The major differentiator between sample groups was calcium microcrystal formation in all ADCT samples, not found in any of the control group samples, which may indicate disc degradation. The incorporation of mineral particles provided clear contrast between the different materials and chemical analysis of a single particle indicated the presence of magnesium-containing calcium phosphate. As IVD calcification is a primary indicator of disc degeneration, further investigation of ADCT and detailed investigations assessing each patient's Pfirrmann degeneration grade following herniation is warranted. Structural phenomena unique to ADCT herniation prompt further investigation of the therapy's mechanisms and its effect on IVD tissue. However, the impossibility of a perfect control group limits the generalizable interpretation of the results.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Rapid Repeated Recurrent Lumbar Disc Herniation after Microscopic Discectomies
- Clinical Analysis of Recurrent Lumbar Disc Herniation
- Surgical Results and Risk Factors for Recurrence of Lumbar Disc Herniation
- Clinical Outcome and Influencing Factor for Repeat Lumbar Discectomy for Ipsilateral Recurrent Lumbar Disc Herniation
- A Case of Scleroderma following Autologous Peripheral Stem Cell Transplantation
