J Korean Ophthalmol Soc.  2018 May;59(5):478-483. 10.3341/jkos.2018.59.5.478.

A New Model of Extraocular Muscle Fibrosis by Thermal Cauterization in the Rats

Affiliations
  • 1Department of Ophthalmology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Korea. njmoon@cau.ac.kr

Abstract

PURPOSE
To evaluate the effect of cauterization on extraocular muscle (EOM) fibrosis in rats, and to develop a novel EOM fibrosis model.
METHODS
Twenty-four eyes of 12 Sprague Dawley rats were assigned randomly to two groups. We exposed the superior rectus muscle (SRM) and performed thermal injury 2 mm behind the insertion site of the SRM using a cautery device in the experimental group. The thermal injuries were performed twice for 1 second, for a total of 2 seconds. In the control group, the same procedures except the thermal injury were performed. Two weeks after surgery, all eyes were enucleated and stained with hematoxylin and eosin (H&E) and Masson's trichrome (MT).
RESULTS
Staining with H&E and MT showed that thermal injury significantly increased inflammation and fibrosis in the experimental group (p=0.002, p < 0.001, respectively).
CONCLUSIONS
Thermal injury using cauterization effectively induced fibrosis of EOM in the rat model. This simple model was effective in inducing fibrosis of SRM and will be useful for studying postoperative fibrosis after strabismus surgery.

Keyword

Cauterization; Extraocular muscle; Fibrosis; Rat; Thermal injury

MeSH Terms

Animals
Cautery*
Eosine Yellowish-(YS)
Fibrosis*
Hematoxylin
Inflammation
Models, Animal
Rats*
Rats, Sprague-Dawley
Strabismus
Eosine Yellowish-(YS)
Hematoxylin

Figure

  • Figure 1. Demonstration of the operation procedure. (A) Exposure of the upper conjunctiva. (B) Subconjunctival injection of 0.1 mL of normal saline. (C) Limbal peritomy from 10 to 2 o'clock. (D) Isolation of the superior rectus muscle. (E) Thermal injury on superior rectus muscle (black arrow).

  • Figure 2. Photomicrographs showing inflammation of the superior rectus muscle two weeks after surgery in the rat model (hematoxylin and eosin staining, ×100). (A) Grade 0. (B) Grade 1. (C) Grade 2. (D) Grade 3.

  • Figure 3. Bar graphs showing the degree of inflammation of superior rectus muscle two weeks after surgery. The grade of inflammation was statistically different between control and cauterization group (p = 0.002).

  • Figure 4. Photomicrographs showing fibrosis of the superior rectus muscle two weeks after surgery in the rat model (Masson's trichrome staining, ×100). (A) Grade 0. (B) Grade 1. (C) Grade 2. (D) Grade 3. (E) Grade 4.

  • Figure 5. Bar graphs showing the degree of fibrosis of superior rectus muscle two weeks after surgery. The grade of fibrosis was statistically different between control and cauterization group (p ≤ 0.001).


Reference

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