Clin Exp Reprod Med.  2017 Mar;44(1):52-55. 10.5653/cerm.2017.44.1.52.

A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser

• Chen H
• Feng G
• Zhang B
• Zhou H
• Shu J
• Gan X

• KMID: 2408588
• DOI: http://doi.org/10.5653/cerm.2017.44.1.52

Abstract

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.

Keyword

Cryopreservation; Immotile spermatozoa; Intracytoplasmic sperm injections; Lasers

MeSH Terms

Cryopreservation
Embryonic Structures
Fertility Preservation
Fertilization
Humans
Male
Oocytes
Pregnancy*
Sperm Injections, Intracytoplasmic
Spermatozoa*
Survival Rate