Ann Lab Med.  2018 Jul;38(4):378-380. 10.3343/alm.2018.38.4.378.

Rapid Identification of bla(IMP-1) and bla(IMP-6) by Multiplex Amplification Refractory Mutation System PCR

Affiliations
  • 1Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan. rnakano@naramed-u.ac.jp
  • 2Center for Infectious Diseases, Nara Medical University, Nara, Japan.
  • 3International University of Health and Welfare, Shioya Hospital, Tochigi, Japan.

Abstract

No abstract available.


MeSH Terms

Polymerase Chain Reaction*

Figure

  • Fig. 1 Representative multiplex ARMS PCR patterns of IMP-1- and IMP-6-producing strains.Abbreviations: ARMS, amplification refractory mutation system; Lane M, 100-bp DNA ladder marker.


Reference

1. Gupta N, Limbago BM, Patel JB, Kallen AJ. Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin Infect Dis. 2011; 53:60–67. PMID: 21653305.
2. Nordmann P, Naas T, Poirel L. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis. 2011; 17:1791–1798. PMID: 22000347.
3. Yano H, Ogawa M, Endo S, Kakuta R, Kanamori H, Inomata S, et al. High frequency of IMP-6 among clinical isolates of metallo-β-lactamase-producing Escherichia coli in Japan. Antimicrob Agents Chemother. 2012; 56:4554–4555. PMID: 22664972.
4. Saito K, Nakano R, Suzuki Y, Nakano A, Ogawa Y, Yonekawa S, et al. Suitability of carbapenem inactivation method (CIM) for detection of IMP metallo-β-lactamase-producing Enterobacteriaceae. J Clin Microbiol. 2017; 55:1220–1222. PMID: 28100600.
5. Yano H, Kuga A, Okamoto R, Kitasato H, Kobayashi T, Inoue M. Plasmid-encoded metallo-β-lactamase (IMP-6) conferring resistance to carbapenems, especially meropenem. Antimicrob Agents Chemother. 2001; 45:1343–1348. PMID: 11302793.
6. Kayama S, Shigemoto N, Kuwahara R, Onodera M, Yokozaki M, Ohge H, et al. Rapid detection of blaIMP-6 by amplification refractory mutation system. J Microbiol Methods. 2012; 88:182–184. PMID: 22108497.
7. Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, et al. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 1989; 17:2503–2516. PMID: 2785681.
8. Nakano R, Nakano A, Hikosaka K, Kawakami S, Matsunaga N, Asahara M, et al. First report of metallo-β-lactamase NDM-5-producing Escherichia coli in Japan. Antimicrob Agents Chemother. 2014; 58:7611–7612. PMID: 25246390.
9. CLSI. Performance standards for antimicrobial susceptibility testing. 22nd ed. Wayne, PA: Clinical and Laboratory Standards Institute;2012. CLSI supplement M100-S22.
Full Text Links
  • ALM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr