Lab Anim Res.  2017 Jun;33(2):49-56. 10.5625/lar.2017.33.2.49.

Quinolone susceptibility and genetic characterization of Salmonella enterica subsp. enterica isolated from pet turtles

• De Silva B
• Hossain S
• Wimalasena SHMP
• Pathirana
• Wendt M
• Heo GJ

• KMID: 2407416
• DOI: http://doi.org/10.5625/lar.2017.33.2.49

Abstract

Turtle-borne Salmonella enterica owns significance as a leading cause in human salmonellosis. The current study aimed to determine the quinolone susceptibility and the genetic characteristics of 21 strains of S. enterica subsp. enterica isolated from pet turtles. Susceptibility of four antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin, and levofloxacin was examined in disk diffusion and MIC tests where the majority of the isolates were susceptible to all tested quinolones. In genetic characterization, none of the isolates were positive for qnr or aac(6')-Ib genes and no any target site mutations could be detected in gyrA, gyrB, and parC quinolone resistance determining regions (QRDR). In addition, neighbor-joining phylogenetic tree derived using gyrA gene sequences exhibited two distinct clads comprising; first, current study isolates, and second, quinolone-resistant isolates of human and animal origin. All results suggest that studied strains of S. enterica subsp. enterica isolated from pet turtles are susceptible to quinolones and genetically more conserved with regards to gyrA gene region.

Keyword

Salmonella enterica subsp. enterica; quinolone susceptibility; pet turtles; qnr genes; QRDR

MeSH Terms

Animals
Ciprofloxacin
Diffusion
Humans
Levofloxacin
Nalidixic Acid
Ofloxacin
Quinolones
Salmonella enterica*
Salmonella Infections
Salmonella*
Trees
Turtles*
Ciprofloxacin
Nalidixic Acid
Ofloxacin
Quinolones

Figure

• 

  Figure 1 Neighbor-joining phylogenetic tree derived by analyzing S. enterica subsp. enterica gyrA sequences obtained from the current study and downloaded from NCBI. Sequences referred to as AJ919282.1, GU330246.1, HQ448882.1, KP184391.1, FJ222660.1, HQ176368.1, KC121321.1, KP290113.1, and EU512996.1 were obtained from NCBI public database and the rest of the sequences were acquired from the current study. 1, 2-Major clads

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