Clin Exp Reprod Med.  2017 Jun;44(2):90-95. 10.5653/cerm.2017.44.2.90.

Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

• Kim HJ
• Yoon HJ
• Jang JM
• Lee WD
• Yoon SH
• Lim JH

• KMID: 2405156
• DOI: http://doi.org/10.5653/cerm.2017.44.2.90

Abstract

OBJECTIVE
We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development.
METHODS
A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination.
RESULTS
No significant differences were observed between the two groups regarding clinical outcomes (p>0.05). ICSI-derived embryos showed significantly faster morphokinetics than those derived from conventional IVF, from the time to pronuclear fading to the time to 6 cells (p<0.05). However, no significant differences were found from the time to 7 cells to the time to expanded blastocyst (p>0.05). There were no differences in abnormal cleavage events between the two groups (p>0.05); they showed the same rates of direct cleavage from 1 to 3 cells, 2 multinucleated cells, 2 uneven cells, and reverse cleavage.
CONCLUSION
The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. However, these differences did not affect the clinical outcomes of the embryo. Additionally, no significant differences in abnormal cleavage events were found according to the fertilization method.

Keyword

In vitro fertilization; Insemination; Intracytoplasmic sperm injection; Time-lapse imaging

MeSH Terms

Blastocyst
Embryonic Development*
Embryonic Structures*
Female
Fertilization
Fertilization in Vitro
Humans*
In Vitro Techniques*
Insemination
Methods
Oocytes*
Ovum
Pregnancy
Sperm Injections, Intracytoplasmic
Spermatozoa*
Time-Lapse Imaging