Korean J Reprod Med.  2009 Dec;36(4):249-254.

A Prospective Comparison of Fertilizability of in vitro Matured Human Oocytes Obtained from Stimulated Cycle: Conventional Versus ICSI

• Jee BC
• An SJ
• Moon JH
• Hwang EJ
• Suh CS
• Kim SH
• Moon SY

Abstract

OBJECTIVE
The aim of this study was to compare the fertilization and cleavage rates of human in vitro matured oocytes after fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).
METHODS
A total of 135 GV stage oocytes were obtained from 59 women who received ovarian stimulation and IVF during Jan 2007 to Oct 2008. Ovarian hyperstimulation was performed using hMG or recombinant FSH with GnRH antagonist and then ovulation triggered by recombinant hCG. The immature oocytes obtained from stimulation cycles were cultured in IVM medium up to 48 hrs; commercial medium supplemented with rFSH 75 mIU/mL, rhCG 0.5 IU/mL and rEGF 10 ng/mL. The in vitro matured oocytes were fertilized by conventional IVF (41 GV oocytes) or ICSI method (94 GV oocytes).
RESULTS
Maturation rate were 51.2% and 59.6% in conventional IVF group and ICSI group, respectively. There was no significant difference in fertilization rates between two groups; 71.4% and 80.4%, respectively. The cleavage rate was also similar in two groups.
CONCLUSION
The presented data suggest that conventional IVF has comparable fertilization and cleavage potential compared with ICSI as the insemination method of immature human oocytes obtained from stimulated cycle.

Keyword

Immature oocyte; in vitro fertilization; Intracytoplasmic sperm injection

MeSH Terms

Female
Fertilization
Fertilization in Vitro
Gonadotropin-Releasing Hormone
Humans
Insemination
Oocytes
Ovulation
Ovulation Induction
Prospective Studies
Sperm Injections, Intracytoplasmic
Gonadotropin-Releasing Hormone