Clin Exp Reprod Med.  2017 Jun;44(2):85-89. 10.5653/cerm.2017.44.2.85.

Influence of the insemination method on the outcomes of elective blastocyst culture

• Wang C
• Feng G
• Zhang B
• Shu J
• Zhou H
• Gan X
• Lin R

• KMID: 2405155
• DOI: http://doi.org/10.5653/cerm.2017.44.2.85

Abstract

OBJECTIVE
The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture.
METHODS
We retrospectively analyzed the outcomes of elective blastocyst culture performed between January 2011 and December 2014.
RESULTS
There were 2,003 cycles of conventional in vitro fertilization (IVF) and 336 cycles of intracytoplasmic sperm injection (ICSI), including 25,652 and 4,164 embryos that underwent sequential blastocyst culture, respectively. No significant differences were found in the female patients' age, basal follicle-stimulating hormone level, basal luteinizing hormone level, body mass index, number of oocytes, maturity rate, fertilization rate, or good-quality embryo rate. However, the blastocyst formation rate and embryo utilization rate were significantly higher in the conventional IVF group than in the ICSI group (54.70% vs. 50.94% and 51.09% vs. 47.65%, respectively, p<0.05). The implantation/pregnancy rate (IVF, 50.93%; ICSI, 55.10%), miscarriage rate (IVF, 12.57%; ICSI, 16.29%), and live birth rate (IVF, 42.12%; ICSI, 44.08%) were similar (p>0.05). No cycles were canceled due to the formation of no usable blastocysts.
CONCLUSION
Although the fertilization method had no effect on clinical outcomes, the blastocyst formation rate and embryo utilization rate in the ICSI group were significantly lower than those observed in the conventional IVF group. Therefore, more care should be taken when choosing to perform blastocyst culture in ICSI patients.

Keyword

Blastocyst; In vitro fertilization; Intracytoplasmic sperm injection; Pregnancy rate

MeSH Terms

Abortion, Spontaneous
Blastocyst*
Body Mass Index
Embryonic Structures
Female
Fertilization
Fertilization in Vitro
Follicle Stimulating Hormone
Humans
Insemination*
Live Birth
Luteinizing Hormone
Methods*
Oocytes
Pregnancy
Pregnancy Rate
Retrospective Studies
Sperm Injections, Intracytoplasmic
Follicle Stimulating Hormone
Luteinizing Hormone