Nat Prod Sci.  2017 Dec;23(4):235-238. 10.20307/nps.2017.23.4.235.

Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control Potential of Essential Oil Isolated from Iranian Cymbopogon citratus Stapf

• Ebadollahi A
• Geranmayeh J
• Kamrani M

• KMID: 2401573
• DOI: http://doi.org/10.20307/nps.2017.23.4.235

Abstract

Colorado potato beetle is a most destructive insect pest of potato throughout the world. Although utilization of chemical insecticides is a main method for management of this pest, their negative side-effects such as threat to humans and the environmental pollution prompted researchers to search for natural alternatives. Recently plant essential oils with low or without side-effects against noun-targeted organisms and with high availability were considered as safe bio-pesticides. In the present study, toxicity of essential oil of Iranian lemongrass, Cymbopogon citratus Stapf, was evaluated against 3th instar larvae and adults of Colorado potato beetle by a leaf dipping method. Results displayed essential oil had notable toxicity against both larvae and adults after 24 and 48 h exposure times. Probit analysis revealed LCâ‚…â‚€ values (lethal concentration to kill 50% of population) with 95% confidence limits were 10.32 (9.17 - 11.72) and 7.76 (6.80 - 8.74) µl/ml for larvae and 6.27 (4.82 - 8.15) and 4.35 (3.24 - 5.62) µl/ml for adults after 24 and 48 h, respectively. Based on regression analysis, a positive correlation between log concentration of essential oil and insect mortality was achieved. Results indicated C. citratus essential oil can be candidate as a natural alternative to the harmful chemical insecticides in the management of Colorado potato beetle.

Keyword

Cymbopogon citratus; Essential oil; Leptinotarsa decemlineata; Toxicity

MeSH Terms

Adult
Beetles*
Colorado*
Cymbopogon*
Environmental Pollution
Humans
Insecticides
Insects
Larva
Methods
Mortality
Oils, Volatile
Plants
Solanum tuberosum*
Insecticides
Oils, Volatile

Figure

• 

  Fig. 1 Toxicity of essential oil of Cymbopogon citratus against the 3th instar larvae (A) and adults (B) of Leptinotarsa decemlineata after 24 and 48 h exposure times. According to Tukey's test, means with the dissimilar letters are significantly different at P = 0.05. There was not any mortality in the control groups.

• 

  Fig. 2 Concentration-response lines for different concentrations of Cymbopogon citratus essential oil against 3th instar larvae and adults of Leptinotarsa decemlineata.

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