J Dent Hyg Sci.  2017 Dec;17(6):495-500. 10.17135/jdhs.2017.17.6.495.

Analgesic Effect of Hippophae rhamnoides Extract in Orofacial Pain in Rats

• Kim YK
• Choi JH
• Kim HJ
• Yoon HS
• Hyun KY
• Lee MK

• KMID: 2401302
• DOI: http://doi.org/10.17135/jdhs.2017.17.6.495

Abstract

Hippophae rhamnoides L. (sea buckthorn) is a shrub wood that belongs to the bamboo tree family, and is rich in vitamin C, D, and E; it is referred to as a vitamin tree. It is mainly grown in the high mountains of Europe and Central Asia, and has been widely used in China and Russia as natural medicine. Recent studies have shown that it is effective in the treatment of cancer, liver diseases, cardiovascular diseases, and gastrointestinal diseases. However, results of studies on its effect on the regulation of pain are insufficient. In this study, we investigated the effect of sea buckthorn on the development and control of pain in two facial areas. The experimental animals included 7- to 8-week-old Sprague-Dawley rats (240~260 g). Formalin (5%), which is known as an inflammation inducer, was injected into the vibrissa pad or temporomandibular joints to induce orofacial acute pain. Rubbing or scraping of the region injected with formalin was regarded as a pain index, and the behavioral response was observed for 45 minutes after the injection. Sea buckthorn extract diluted to 150, 300 mg/kg (in 1 ml of distilled water) was orally administered 30 minutes prior to the acute pain. The facial pain behavior was effectively reduced in the 300 mg/kg group when compared to the control group (vehicle). Likewise, in an experiment in which formalin was injected into the temporomandibular joints, effective pain alleviation was confirmed at the same drug concentration. These results suggest that sea buckthorn extract may be useful in the development of therapeutic agents for acute inflammatory pain in the orofacial area and for controlling temporomandibular joint pain.

Keyword

Hippophae; Orofacial pain; Temporomandibular joint

MeSH Terms

Acute Pain
Animals
Ascorbic Acid
Asia
Cardiovascular Diseases
China
Europe
Facial Pain*
Formaldehyde
Gastrointestinal Diseases
Hippophae*
Humans
Inflammation
Liver Neoplasms
Rats*
Rats, Sprague-Dawley
Russia
Temporomandibular Joint
Trees
Vitamins
Wood
Ascorbic Acid
Formaldehyde
Vitamins