J Periodontal Implant Sci.  2017 Dec;47(6):388-401. 10.5051/jpis.2017.47.6.388.

Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications

Affiliations
  • 1School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Korea. jhlee7@skku.edu, kimdj@skku.edu
  • 2SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, Korea.

Abstract

PURPOSE
The physicochemical properties of a xenograft are very important because they strongly influence the bone regeneration capabilities of the graft material. Even though porcine xenografts have many advantages, only a few porcine xenografts are commercially available, and most of their physicochemical characteristics have yet to be reported. Thus, in this work we aimed to investigate the physicochemical characteristics of a porcine bone grafting material and compare them with those of 2 commercially available bovine xenografts to assess the potential of xenogenic porcine bone graft materials for dental applications.
METHODS
We used various characterization techniques, such as scanning electron microscopy, the Brunauer-Emmett-Teller adsorption method, atomic force microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and others, to compare the physicochemical properties of xenografts of different origins.
RESULTS
The porcine bone grafting material had relatively high porosity (78.4%) and a large average specific surface area (SSA; 69.9 m²/g), with high surface roughness (10-point average roughness, 4.47 µm) and sub-100-nm hydroxyapatite crystals on the surface. Moreover, this material presented a significant fraction of sub-100-nm pores, with negligible amounts of residual organic substances. Apart from some minor differences, the overall characteristics of the porcine bone grafting material were very similar to those of one of the bovine bone grafting material. However, many of these morphostructural properties were significantly different from the other bovine bone grafting material, which exhibited relatively smooth surface morphology with a porosity of 62.0% and an average SSA of 0.5 m²/g.
CONCLUSIONS
Considering that both bovine bone grafting materials have been successfully used in oral surgery applications in the last few decades, this work shows that the porcine-derived grafting material possesses most of the key physiochemical characteristics required for its application as a highly efficient xenograft material for bone replacement.

Keyword

Bioprosthesis; Chemical phenomena; Dental materials; Durapatite; Heterografts

MeSH Terms

Adsorption
Bioprosthesis
Bone Regeneration
Bone Transplantation
Chemical Phenomena
Dental Materials
Durapatite
Heterografts*
Methods
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Porosity
Spectrum Analysis
Surgery, Oral
Transplants*
X-Ray Diffraction
Dental Materials
Durapatite

Figure

  • Figure 1 Size distribution of the examined xenografts.

  • Figure 2 Surface morphologies of the xenograft materials. (A-C) THE Graft, (D-F) Bio-Oss®, and (G-I) Cerabone®.

  • Figure 3 Pore size distribution of the graft materials.

  • Figure 4 Measurement of the roughness of the grafting materials using 3D AFM. (A) The 2D AFM image of porcine graft section cut with an ultramicrotome. The 3D tomography of (B) THE Graft and (C) Bio-Oss® was obtained by stacking their 2D AFM images. The RzJIS roughness of the samples from THE Graft and Bio-Oss® was found to be 4.47 and 1.37 µm, respectively. 3D: 3-dimensional, 2D: 2-dimensional, AFM: atomic force microscopy, RzJIS: Japanese Industrial Standard for roughness, HA: hydroxyapatite.

  • Figure 5 XRD patterns of THE Graft, Bio-Oss®, and Cerabone®. The blue dots mark the reference hydroxyapatite peaks. XRD, X-ray diffraction.

  • Figure 6 FT-IR spectra of (A) THE Graft, (B) Bio-Oss®, and (C) Cerabone®. FT-IR, fourier-transform infrared.

  • Figure 7 Wetting mass of the graft materials as a function of time.


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