J Periodontal Implant Sci.  2017 Dec;47(6):381-387. 10.5051/jpis.2017.47.6.381.

Ridge preservation using basic fibroblast growth factor-2 and collagenated biphasic calcium phosphate in beagle dogs

• Sohn B
• Hwang M
• Kim S
• Kim HI
• Ku Y

• KMID: 2399744
• DOI: http://doi.org/10.5051/jpis.2017.47.6.381

Abstract

PURPOSE
The aim of this study was to evaluate volumetric and histologic changes in edentulous alveolar ridge areas after ridge preservation using basic fibroblast growth factor-2 (bFGF-2) in combination with collagenated biphasic calcium phosphate (BCP).
METHODS
The experiments were performed in 6 adult male beagle dogs. The following 3 groups were created: 1) ridge preservation with bFGF-2 and collagenated BCP (experimental group), 2) ridge preservation with collagenated BCP (positive control group), and 3) a negative control group in which no ridge preservation procedure was performed. Volumetric change analysis was performed using an optical scanner and casts. Histological observations were made using light microscopy.
RESULTS
After the initial swelling subsided, the magnitude of the volumetric change in the experimental group and positive control group was smaller than in the negative control group. In the experimental group, a distinct trend was observed for the resorption of residual bone and collagen fibers at 4 weeks and for more mature bone and faster healing at 12 weeks.
CONCLUSIONS
Based on the findings of the present study, bFGF-2 may be considered for use as a therapeutic molecule in ridge preservation procedures.

Keyword

Alveolar process; Fibroblast growth factors; Hydroxyapatite-beta tricalcium phosphate; Tooth extraction

MeSH Terms

Adult
Alveolar Process
Animals
Calcium*
Collagen*
Dogs*
Fibroblast Growth Factor 2*
Fibroblast Growth Factors
Fibroblasts*
Humans
Male
Microscopy
Tooth Extraction
Calcium
Collagen
Fibroblast Growth Factor 2
Fibroblast Growth Factors

Figure

• 

  Figure 1 Surgical procedure. Six beagle dogs and 24 extraction sockets were involved. The maxillary second premolars and third premolars were extracted. Twelve teeth were extracted at the first intervention and other 12 teeth were extracted at the second intervention. Impressions were taken at 2-, 4-, and 12-weeks after surgery. The dogs were sacrificed 12 weeks after the first intervention.

• 

  Figure 2 Analysis of volumetric changes. (A) Intraoral image and ROI. (B) Three-dimensional scan image and ROI. (C) Superimposition of the 2 images. (D) Projection of the ROI. ROI: region of interest.

• 

  Figure 3 The ROI was drawn in the coronal portion of the socket (A: bar=1 mm, B: bar=500 μm, C: bar=100 μm). ROI: region of interest

• 

  Figure 4 Histologic evaluation. (A) Negative control, 4 weeks: a large portion of the socket was composed of fibrous connective tissue. (B) Positive control, 4 weeks: woven bone had formed and was lined by osteoblasts. Some osteoclasts were identified near graft particles. (C) Experimental group, 4 weeks: there was distinct resorption of residual graft particles. Woven bone from the existing bone was formed where enhanced angiogenesis was observed. (D) Negative control, 12 weeks: a limited amount of new bone was observed. The woven bone was lined by inactive and flat osteoblasts. (E) Positive control, 12 weeks: osteocytes were found in the newly formed bone and bone maturation had progressed. (F) Experimental group, 12 weeks: new bone formation and angiogenesis were evident. More mature bone and faster healing were found (A–F: bar=100 μm). Arrowheads, arrow, and asterisk indicate osteoclasts, osteoblasts, and vessels, respectively.

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