Korean J Physiol Pharmacol.  2018 Jan;22(1):17-21. 10.4196/kjpp.2018.22.1.17.

Morroniside on anti-inflammation activities in rats following acute myocardial infarction

• Yu B
• Zhang G
• An Y
• Wang W

• KMID: 2398551
• DOI: http://doi.org/10.4196/kjpp.2018.22.1.17

Abstract

Our previous studies have confirmed that morroniside has neuroprotective effects. However, the effects of morroniside on cardiac myocardium remain unknown. Rats were anaesthetized with 10% chloral hydrate (0.35~0.4 mL/kg) and an acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD). Following AMI, morroniside was administered intragastrically for 3 consecutive days at doses of 45, 90 and 180 mg/kg, respectively. Lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) activities in AMI rats in the serum were detected with commercial kits. The expression of IL-6, IL-1β and TNF-α in myocardium was detected by Western blotting analysis. We observed a significant decline in the Q(q) wave amplitude in morroniside-treated rats after 72 h. Additionally, treatment of morroniside decreased the levels of LDH and cTnT in AMI rats. We also observed that morroniside reduced the expression of IL-6, IL-1β and TNF-α in myocardium. Taken together, our findings demonstrate that morroniside had effective anti-inflammatory properties in AMI rats.

Keyword

Acute myocardial infarction; Cytokine; Inflammation; Morroniside

MeSH Terms

Animals
Blotting, Western
Chloral Hydrate
Coronary Vessels
Inflammation
Interleukin-6
L-Lactate Dehydrogenase
Myocardial Infarction*
Myocardium
Neuroprotective Agents
Rats*
Troponin T
Chloral Hydrate
Interleukin-6
L-Lactate Dehydrogenase
Neuroprotective Agents
Troponin T

Figure

• 

  Fig. 1 Structure of morroniside.

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  Fig. 2 Electrocardiogram (lead II).(A) Normal (sham) ECG, (B) 5 min post AMI, (C) Model, 72 h post AMI, (D) Morroniside-treated, 72 h post AMI, (E) Amplitude of Q(q) wave (ECG) post AMI 72 h (model and morroniside-treated AMI rats). n=8 per group, *p<0.05 vs. model.

• 

  Fig. 3 Effects of morroniside on the activities of LDH and cTnT in the serum in acute myocardial infarction (AMI) rats.n=8 per group, *p<0.05 vs. model. #p<0.05 vs. sham.

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  Fig. 4 Western blotting analysis of IL-6, IL-1β and TNF-α.GAPDH was used as an internal control. Densitometry for IL-6, IL-1β and TNF-α expression normalized to GAPDH. n=8 per group, *p<0.05 vs. model. #p<0.05 vs. sham.

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