J Korean Acad Oral Health.  2017 Sep;41(3):188-193. 10.11149/jkaoh.2017.41.3.188.

In vitro antimicrobial activity of different mouthwashes available in Korea

• You MS
• Lee SY
• Ma DS

• KMID: 2392437
• DOI: http://doi.org/10.11149/jkaoh.2017.41.3.188

Abstract

OBJECTIVES
The purpose of this study was to compare the oral antimicrobial effects of seven different mouthwashes available in Korea.
METHODS
To examine the antimicrobial effects of the seven mouthwashes, their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined using broth microdilution methods. Streptococcus mutans ATCC 25175, Lactobacillus acidophilus ATCC 4355, Candida albicans KCTC 7270, and Porphyromonas gingivalis ATCC 33277 were used in this experiment. S. mutans and P. gingivalis were examined using a scanning electron microscope (SEM), after treatment with the mouthwashes containing cetylpyridinium chloride (CPC).
RESULTS
Mouthwashes containing CPC had lower MIC and MBC values against the four microorganisms. Their bactericidal effects were concentration-dependent. S. mutans and C. albicans were highly sensitive to the concentration of CPC in the mouthwashes. According to the SEM observation, the treatment of bacteria with mouthwashes containing CPC, changed the cell surface texture of S. mutans and P. gingivalis.
CONCLUSIONS
Mouthwashes containing CPC showed relatively lower MIC and MBC values under the same conditions against the four microorganisms used in this study.

Keyword

Antimicrobial activity; Mouthwash; Oral microorganisms

MeSH Terms

Bacteria
Candida albicans
Cetylpyridinium
In Vitro Techniques*
Korea*
Lactobacillus acidophilus
Mouthwashes*
Porphyromonas gingivalis
Streptococcus mutans
Cetylpyridinium
Mouthwashes

Figure

• 

  Fig. 1 Effects of MW1 on bacterial killing by concentration. Serially diluted mouth wash was added to wells containing bacteria and incubated for 5 min at 37℃ to assess the effects of the mouthwash concentration. After incubation, each sample was serially diluted and the diluents were spread on agar plates. The plates were incubated for 2-5 days and the number of CFUs was measured. The values are the means of duplicate determinations and the error bars indicate standard deviations of the mean

• 

  Fig. 2 Scanning electron microscopy (SEM). S. mutans and P. gingivalis were treated with MW1 mouthwash solution for 1 min and 60 min at 25℃. The bacterial suspensions after treatment were centrifuged and bacterial pellets were fixed in glutaraldehyde in PBS. The fixed samples were then washed and dehydrated in a graded series of ethanol, substituted with a series of isoamyl acetate. After critical point drying, the samples were mounted on stub, coated with gold and observed with SEM.

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