Nutr Res Pract.  2009 Dec;3(4):259-264.

Loquat (Eriobotrya japonica) extracts suppress the adhesion, migration and invasion of human breast cancer cell line

• Kim MS
• You MK
• Rhuy DY
• Kim YJ
• Baek HY
• Kim HA

Abstract

We examined the inhibitory effects of loquat methanol extract on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MDA-MB-231 human breast cancer cell line. Cells were cultured with DMSO or with 10, 25, or 50 microg/ml of loquat methanol extract. Both leaf and seed extracts significantly inhibited growth of MDA-MB-231 cells in a dose-dependent manner, although leaf extract was more effective. Adhesion and migration were significantly inhibited by loquat extracts in a dose-dependent manner. Loquat extract also inhibited the invasion of breast cancer cells in a dose-dependent manner and leaf extract was more effective than seed extract. MMP-2 and MMP-9 activities were also inhibited by loquat extract. Our results indicate that methanol extracts of loquat inhibit the adhesion, migration and invasion of human breast cancer cells partially through the inhibition of MMP activity and leaf extract has more anti-metastatic effects in cell based assay than seed extract. Clinical application of loquat extract as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

Keyword

Loquat (Eriobotrya japonica); adhesion; migration; invasion; MMP

MeSH Terms

Breast Neoplasms
Cell Line
Dimethyl Sulfoxide
Eriobotrya
Humans
Methanol
Neoplasm Metastasis
Seeds
Dimethyl Sulfoxide
Methanol

Figure

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  Fig. 1 Effect of Eriobotrya japonica Lindley extracts on MDA-MB231 cell proliferation. Cells were seeded in 6-well culture plates at a concentration of 6×104/well. Complete medium with DMSO or with 10, 25 or 50 µg/ml of leaf or seed extract were replaced every the other day. Cells cultured in complete medium were harvested on day 5 after treatment and were counted. Data were expressed as mean ± SD. Stand error bars represent three independent experiments and each experiment was triplicated. Means with the same letter are not significantly different by Duncan's multiple test (P < 0.05).

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  Fig. 2 Effect of Eriobotrya japonica Lindley extracts on MDA-MB231 cell adhesion. Cells were added to matrigel coated plates and incubated for 90 min in present of DMSO or 10, 25 or 50 µg/ml of leaf or seed extract. Attached cells were counted. Data were expressed as mean ± SD. Stand error bars represent three independent experiments and each experiment was triplicated. Means with the same letter are not significantly different by Duncan's multiple test (P < 0.05).

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  Fig. 3 Effect of Eriobotrya japonica Lindley extracts on MDA-MB231 cell migration. Cells were seeded into 6 well cell culture plates and cultured in 10% FBS DMEM to near confluence. Confluent monolayer was carefully wounded and washed cellular debris gently with PBS. The wounded monolayer was incubated in 10% FBS DMEM containing 20 µg/ml fibronectin and DMSO or, 10, 25 or 50 µg/ml of leaf or seed extract for 24 hours.

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  Fig. 4 Effect of Eriobotrya japonica Lindley extracts on MDA-MB231 cell invasion (P < 0.05). Boyden chamber was used for the invasion assay. Cells were treated with DMSO or 10, 25 or 50 µg/ml of leaf or seed extract for 24 hours during assay. Stand error bars represent three independent experiments and each experiment was triplicated. Means with the same letter are not significantly different by Ducan's multiple test (P < 0.05).

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  Fig. 5 Effects of Eriobotrya japonica Lindley on the activities of MMP2 and MMP9 of MDA-MB231 cells. 1: DMSO 2: seed10 µg/ml 3: seed 25 µg/ml 4: seed 50 µg/ml 5: leaves 10 µg/ml 6: leaves 25 µg/ml 7: leaves 50 µg/ml

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