Gut Liver.  2017 Sep;11(5):648-654. 10.5009/gnl16503.

In Vitro Activity of Diphenyleneiodonium toward Multidrug-Resistant Helicobacter pylori Strains

• Chung JW
• Kim SY
• Park HJ
• Chung CS
• Lee HW
• Lee SM
• Kim I
• Pak JH
• Lee GH
• Jeong JY

• KMID: 2389353
• DOI: http://doi.org/10.5009/gnl16503

Abstract

BACKGROUND/AIMS
The increased resistance of Helicobacter pylori to antibiotics has increased the need to develop new treatments for this bacterium. The aim of our study was to identify new drugs with anti-H. pylori activity.
METHODS
We screened a small molecule library"”the library of pharmacologically active compounds (LOPAC), which includes 1,280 pharmacologically active compounds"”to identify inhibitors of H. pylori growth. The minimal inhibitory concentrations (MICs) of antibiotics against multidrug-resistant H. pylori strains were determined using the agar dilution method.
RESULTS
We identified diphenyleneiodonium (DPI) as a novel anti-H. pylori agent. The MIC values for DPI were <0.03 μg/mL against all tested H. pylori strains. DPI also exhibited strong antibacterial activity against common gram-negative and gram-positive pathogenic bacteria.
CONCLUSIONS
DPI may be a candidate anti-H. pylori drug for future development.

Keyword

Helicobacter pylori; Diphenyleneiodonium; Drug resistance, multiple; Anti-bacterial agents; Minimal inhibitory concentration

MeSH Terms

Agar
Anti-Bacterial Agents
Bacteria
Drug Resistance, Multiple
Helicobacter pylori*
Helicobacter*
In Vitro Techniques*
Methods
Agar
Anti-Bacterial Agents