Biomol Ther.  2017 Jul;25(4):362-366. 10.4062/biomolther.2017.066.

Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

• Lee EH
• Park CH

• KMID: 2386901
• DOI: http://doi.org/10.4062/biomolther.2017.066

Abstract

Direct conversion by trans-differentiation is of growing interest in cell therapy for incurable diseases. The efficiency of cell reprogramming and functionality of converted cells are important considerations in cell transplantation therapy. Here, we compared two representative protocols for the generation of induced-oligodendrocyte progenitor cells (iOPCs) from mouse and rat fibroblasts. Then, we showed that induction of Nkx6.2, Olig2, and Sox10 (NOS) was more effective in mouse fibroblasts and that induction of Olig2, Sox10, and Zfp536 (OSZ) was more effective at reprogramming iOPCs from rat fibroblasts. However, OSZ-iOPCs did not show greater proliferation than NOS-induced cells. Because the efficiency of iOPCs generation appears to differ between cell species depending on transcription factors and culture conditions, it is important to select appropriate methods for efficient reprogramming.

Keyword

Direct conversion; Oligodendrocyte; iOPC; Efficiency

MeSH Terms

Animals
Cell Transplantation
Cell- and Tissue-Based Therapy
Cellular Reprogramming
Fibroblasts
Methods*
Mice
Oligodendroglia
Rats
Stem Cells
Transcription Factors
Transplants
Transcription Factors