Mycobiology.  2017 Jun;45(2):105-109. 10.5941/MYCO.2017.45.2.105.

Development and Molecular Characterization of Novel Polymorphic Genomic DNA SSR Markers in Lentinula edodes

• Moon S
• Lee HY
• Shim D
• Kim M
• Ka KH
• Ryoo R
• Ko HG
• Koo CD
• Chung JW
• Ryu H

• KMID: 2385659
• DOI: http://doi.org/10.5941/MYCO.2017.45.2.105

Abstract

Sixteen genomic DNA simple sequence repeat (SSR) markers of Lentinula edodes were developed from 205 SSR motifs present in 46.1-Mb long L. edodes genome sequences. The number of alleles ranged from 3-14 and the major allele frequency was distributed from 0.17-0.96. The values of observed and expected heterozygosity ranged from 0.00-0.76 and 0.07-0.90, respectively. The polymorphic information content value ranged from 0.07-0.89. A dendrogram, based on 16 SSR markers clustered by the paired hierarchical clustering' method, showed that 33 shiitake cultivars could be divided into three major groups and successfully identified. These SSR markers will contribute to the efficient breeding of this species by providing diversity in shiitake varieties. Furthermore, the genomic information covered by the markers can provide a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection in the shiitake mushroom.

Keyword

gDNA-SSR; genetic diversity; Lentinula edodes; UPGMA

MeSH Terms

Alleles
Breeding
DNA*
Gene Frequency
Genetic Linkage
Genetic Variation
Genome
Lentinula*
Methods
Microsatellite Repeats
Shiitake Mushrooms*
DNA