Int J Stem Cells.  2017 May;10(1):48-59. 10.15283/ijsc16063.

Cardioprotective Effects of Wharton Jelly Derived Mesenchymal Stem Cell Transplantation in a Rodent Model of Myocardial Injury

Affiliations
  • 1Department of Clinical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. tagrid8@yahoo.com
  • 2Department of Pediatric Cardiology, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • 3Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.

Abstract

BACKGROUND
Whartons jelly-derived mesenchymal stem cells are a valuable alternative source that possess multipotent properties, easy to obtain and available in large scale compared to BMMSCs. We investigated the possibility of cardiac function improvement post isoproterenol induced cardiac injury in a rat model following human WJMSCs transplantation.
MATERIALS AND METHODS
MSCs were extracted and cultured from cord WJ, characterized by morphology, Immunophenotyping and differentiation to osteoblast and adipocytes. WJMSCs were labeled with PKH2 linker dye. Wistar rats were divided into control group, ISO group (injected with 2 doses of isoproterenol) to induce myocardial injury and ISO group transplanted with labelled WJMSCs. ECG, electrocardiographic patterns, cardiac marker enzymes, tracing of labeled MSCs and immunohistochemical analysis of myocardial cryosections were studied.
RESULTS
AND CONCLUSIONS: WJ derived MSCs were expanded for more than 14 passages while maintaining their un-differentiated state, were positive for MSC markers and were able to differentiate into adipocyte and osteoblast. We demonstrated that intravenously administered WJMSCs were capable of homing predominently in the ischemic myocardium. Cardiac markers were positively altered in stem cell treated group compared to ISO group. ECG and ECHO changes were improved with higher survival rate. WJMSCs could differentiate into cardiac-like cells (positive for cardiac specific proteins) in vivo. WJMSCs infusion promoted cardiac protection and reduced mortality, emphasizing a promising therapeutic role for myocardial insufficiency.

Keyword

Mesenchymal stem cells; Isoproterenol; Transplantation; Wharton's jelly; Cardiac repair

MeSH Terms

Adipocytes
Electrocardiography
Humans
Immunophenotyping
Isoproterenol
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stromal Cells*
Models, Animal
Mortality
Myocardium
Osteoblasts
Rats, Wistar
Rodentia*
Stem Cells
Survival Rate
Transplantation
Wharton Jelly*
Isoproterenol
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