Biomol Ther.
2015 Sep;23(5):400-406. 10.4062/biomolther.2015.010.
Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid
- Affiliations
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- 1Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410000, China.
- 2Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China. ygp9880@126.com
- 3Institute of Clinical Pharmacology, Central South University, Changsha, Hunan 410013, China.
- KMID: 2380287
- DOI: http://doi.org/10.4062/biomolther.2015.010
Abstract
- MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
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