Restor Dent Endod.  2017 May;42(2):87-94. 10.5395/rde.2017.42.2.87.

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

• Cruz-Filho AMd
• Bordin AR
• Souza-Flamini LE
• Guedes DF
• Saquy PC
• Silva RG
• Pécora JD

• KMID: 2379457
• DOI: http://doi.org/10.5395/rde.2017.42.2.87

Abstract

OBJECTIVES
Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution.
MATERIALS AND METHODS
Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05).
RESULTS
There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment.
CONCLUSIONS
Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Keyword

Chelators; Chitosan; Colorimetry; Dentin microhardness; Shelf life

MeSH Terms

Calcium
Chelating Agents
Chitosan*
Colorimetry*
Dentin*
Edetic Acid
Glass
Humans
Ions
Plastics
Product Packaging*
Water
Calcium
Chelating Agents
Chitosan
Edetic Acid
Ions
Plastics
Water

Figure

• 

  Figure 1 Schematic representation of the operative sequence for specimen preparation: removal of the dental crown; toot embedded in resin block for section of the cervical portion; cutting of the three cervical sections, the first being discarded; bisection of the second and third cuts in half; inclusion of the cuts on the silicone rings to fill with acrylic resin; specimen. CEJ, cementoenamel junction.

• 

  Figure 2 Ultraviolet-visible (UV/Vis) spectrophotometry of chitosan solutions stored in plastic bottles at different times. It can be observed the 206 nm wavelength relative to the calcium carbonate, indicating the presence of calcium ions in the solution. The 234 nm wavelength for chitosan at all times indicates absence of calcium ions.

• 

  Figure 3 Ultraviolet-visible (UV/Vis) spectrophotometry of chitosan solutions stored in glass bottles at different times. The 234 nm wavelength for the chitosan solution at all times indicates absence of calcium ions.

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