Korean J Parasitol.
2017 Apr;55(2):213-218. 10.3347/kjp.2017.55.2.213.
Interaction between Trichomonas vaginalis and the Prostate Epithelium
- Affiliations
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- 1Department of Environmental Biology and Medical Parasitology, Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea. jsryu@hanyang.ac.kr
- 2Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea.
- 3Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
- 4Department of Microbiology and Immunology, Eulji University College of Medicine, Daejeon 35233, Korea.
- KMID: 2379426
- DOI: http://doi.org/10.3347/kjp.2017.55.2.213
Abstract
- Most men infected with Trichomonas vaginalis are asymptomatic and can remain undiagnosed and untreated. This has been hypothesized to result in chronic persistent prostatic infection. Adhesion of the protozoan organisms to mucosal cells is considered a first and prerequisite step for T. vaginalis infection. Adhesion of T. vaginalis to prostate epithelial cells has not yet been observed; however, there are several reports about inflammation of prostate epithelial cells induced by T. vaginalis. The aim of this study was to investigate whether adhesion and cytotoxicity of T. vaginalis are involved in inflammation of prostate epithelial cells. When RWPE-1 cells were infected with T. vaginalis (1:0.4 or 1:4), adhesion of T. vaginalis continuously increased for 24 hr or 3 hr, respectively. The cytotoxicity of prostate epithelial cells infected with T. vaginalis (RWPE-1: T. vaginalis=1:0.4) increased at 9 hr; at an infection ratio of 1:4, cytotoxicity increased after 3 hr. When the RWPE-1 to T. vaginalis ratio was 1:0.4 or 1:4, production of IL-1β, IL-6, CCL2, and CXCL8 also increased. Epithelial-mesenchymal transition (EMT) was verified by measuring decreased E-cadherin and increased vimentin expression at 24 hr and 48 hr. Taken together, the results indicate that T. vaginalis adhered to prostate epithelial cells, causing cytotoxicity, pro-inflammatory cytokine production, and EMT. Our findings suggest for the first time that T. vaginalis may induce inflammation via adhesion to normal prostate epithelial cells.
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