Lab Anim Res.  2017 Mar;33(1):24-31. 10.5625/lar.2017.33.1.24.

Effects of coenzyme Q₁₀ on the antioxidant system in SD rats exposed to lipopolysaccharide-induced toxicity

• Song MH
• Kim HN
• Lim Y
• Jang IS

• KMID: 2375269
• DOI: http://doi.org/10.5625/lar.2017.33.1.24

Abstract

The study was performed to see the effects of coenzyme Q₁₀ (CoQ₁₀) on blood biochemical components and hepatic antioxidant system in rats exposed to lipopolysaccharide (LPS)-induced toxicity. A total of 24 rats were allocated to four groups: control (CON), 100 mg/kg BW of LPS (LPS), 100 mg of CoQ₁₀/kg BW with LPS (LCQI) and 300 mg of CoQ₁₀/kg BW with LPS (LCQII). The LPS and LCQI groups showed a significant (P<0.05) increase in the relative spleen weight compared with the CON group without affecting body and liver weights. The blood alanine aminotransferase (ALT) level in the LPS group was significantly (P<0.05) greater than that in the CON group, while supplementation with 100 or 300 mg CoQ₁₀ to rats injected with LPS normalized the ALT level in the CON group. In antioxidant systems, the LPS group showed a significantly (P<0.05) higher mRNA and activity of superoxide dismutase (SOD) than the CON group. The supplementation with CoQ₁₀ to the LPS-treated group normalized the level of SOD, which was comparable to the level of the CON group. Both the mRNA expression and activity of glutathione peroxidase in the LCQI and LCQII groups were higher (P<0.05) than that of the LPS group. However, administration of LPS or CoQ₁₀ unaffected the level of catalase and total antioxidant power. The level of lipid peroxidation in the LCQII group was lower (P<0.05) than that in the LPS group. In conclusion, CoQ₁₀ exerted its favorable effect against liver damage by modulation of antioxidant enzymes in LPS treated rats.

Keyword

Coenzyme Q₁₀; lipopolysaccharide; antioxidant enzymes; lipid peroxidation

MeSH Terms

Alanine Transaminase
Animals
Catalase
Glutathione Peroxidase
Lipid Peroxidation
Liver
Rats*
RNA, Messenger
Spleen
Superoxide Dismutase
Weights and Measures
Alanine Transaminase
Catalase
Glutathione Peroxidase
RNA, Messenger
Superoxide Dismutase

Figure

• 

  Figure 1 Specific activity of antioxidant enzymes (A: SOD, B: GPX and C: CAT) in the liver of SD rats administrated saline (CON), LPS injection (LPS), 100 mg of CoQ10/kg BW with injection of LPS (LCQI) and 300 mg of CoQ10/kg BW with injection of LPS (LCQII). Means (Mean±SD, n=6) with different superscript differ among groups (P<0.05).

• 

  Figure 2 The levels of MDA (A) in microsomal fraction of the liver and total antioxidant power (B) in plasma of SD rats administrated saline (CON), LPS injection (LPS), 100 mg of CoQ10/kg BW with injection of LPS (LCQI) and 300 mg of CoQ10/kg BW with injection of LPS (LCQII). Means (Mean±SD, n=6) with different superscript differ among groups (P<0.05).

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