Biomol Ther.  2017 Mar;25(2):194-201. 10.4062/biomolther.2016.046.

Calcium Signaling of Lysophosphatidylethanolamine through LPA1 in Human SH-SY5Y Neuroblastoma Cells

• Lee JM
• Park SJ
• Im DS

• KMID: 2375040
• DOI: http://doi.org/10.4062/biomolther.2016.046

Abstract

Lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, has been reported to be an intercellular signaling molecule. LPE mobilizes intracellular Ca²âº through G-protein-coupled receptor (GPCR) in some cells types. However, GPCRs for lysophosphatidic acid (LPA) were not implicated in the LPE-mediated activities in LPA GPCR overexpression systems or in SK-OV3 ovarian cancer cells. In the present study, in human SH-SY5Y neuroblastoma cells, experiments with LPA₁ antagonists showed LPE induced intracellular Ca²âº increases in an LPA₁ GPCR-dependent manner. Furthermore, LPE increased intracellular Ca²âº through pertussis-sensitive G proteins, edelfosine-sensitive-phospholipase C, 2-APB-sensitive IP₃ receptors, Ca²âº release from intracellular Ca²âº stores, and subsequent Ca²âº influx across plasma membranes, and LPA acted on LPA₁ and LPAâ‚‚ receptors to induce Ca²âº response in a 2-APB-sensitive and insensitive manner. These findings suggest novel involvements for LPE and LPA in calcium signaling in human SH-SY5Y neuroblastoma cells.

Keyword

Lysophosphatidylethanolamine; LPA₁; Lysophosphatidic acid; GPCR; Neuroblastoma; Receptor

MeSH Terms

Calcium Signaling*
Calcium*
Cell Membrane
GTP-Binding Proteins
Humans*
Neuroblastoma*
Ovarian Neoplasms
Calcium
GTP-Binding Proteins