Ann Lab Med.  2017 Mar;37(2):166-168. 10.3343/alm.2017.37.2.166.

A Case of Therapy-Related Acute Leukemia With Mixed Phenotype With BCR-ABL1 After Treatment of Diffuse Large B-Cell Lymphoma

Affiliations
  • 1Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea. mihakimp@hanmail.net
  • 2Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea.
  • 3Department of Internal Medicine, Kosin University College of Medicine, Busan, Korea.
  • 4Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.

Abstract

No abstract available.


MeSH Terms

Aged
Antineoplastic Combined Chemotherapy Protocols/adverse effects
Bone Marrow Cells/cytology/pathology
Female
Flow Cytometry
Fusion Proteins, bcr-abl/*genetics
Granulocyte Colony-Stimulating Factor/therapeutic use
Humans
Immunophenotyping
Leukemia/*diagnosis/etiology
Lymphoma, Large B-Cell, Diffuse/*drug therapy
Phenotype
Rituximab/administration & dosage
Fusion Proteins, bcr-abl
Granulocyte Colony-Stimulating Factor
Rituximab

Figure

  • Fig. 1 Bone marrow aspirate smear showing leukemic blasts. (A) Increased blasts with medium cell size, oval to round shape, vesicular nuclei, fine chromatin patterns, and basophilic cytoplasm (×1,000, Wright-Giemsa stain), (B) More than 3% of blasts were positive for myeloperoxidase (×1,000, myeloperoxidase stain).

  • Fig. 2 Flow cytometry analysis of the bone marrow cells (Cytomics FC500 flow cytometer, Beckman Coulter, Fullerton, CA, USA). By CD45/SS linear gating, a relatively homogenous population of blasts consist 38.46% of all bone marrow cells. These cells were positive for both T-lymphoid (cytoplasmic CD3, CD5, and CD7) and myeloid differentiation (cytoplasmic MPO, CD13, and CD33) markers.Abbreviations: FITC, fluorescein isothiocyanate; MPO, myeloperoxidase.


Reference

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