Ann Lab Med.  2016 Jan;36(1):82-84. 10.3343/alm.2016.36.1.82.

Bone Marrow Chimerism Detection Using Next Generation Sequencing Based on Single Nucleotide Polymorphisms Following Liver Transplantation: Comparison With Short Tandem Repeat-PCR

• Kim J
• Hwang IS
• Kim HS
• Joo DJ
• Hong KR
• Choi JR

• KMID: 2373506
• DOI: http://doi.org/10.3343/alm.2016.36.1.82

Abstract

No abstract available.

MeSH Terms

Adult
Bone Marrow/*pathology
Fatal Outcome
Graft vs Host Disease/etiology
High-Throughput Nucleotide Sequencing
Humans
Liver Cirrhosis/pathology/*therapy
*Liver Transplantation/adverse effects
Microsatellite Repeats
Middle Aged
Polymerase Chain Reaction
*Polymorphism, Single Nucleotide
Transplantation Chimera/*genetics

Figure

• 

  Fig. 1 NGS chimerism analysis 41 days after liver transplant. Thirty-two of ninety SNPs discriminated between the donor and recipient. The average percent of donor DNA was 95.1%. Ninety SNPs are shown for (A) the recipient (peripheral blood, pre-transplant), (B) donor (peripheral blood), and (C) recipient (bone marrow, post-transplant) with mean coverage depth for each SNPs, 674, 1,609 and 1,080, respectively. The following markers were used for the chimerism calculation: rs560681, rs891700, rs12997453, rs6444724, rs2046361, rs159606, rs251934, rs338882, rs13218440, rs727811, rs10092491, rs2056277, rs1463729, rs735155, rs740598, rs964681, rs2076848, rs2269355, rs2111980, rs1058083, rs354439, rs873196, rs2016276, rs1382387, rs9905977, rs2292972, rs1736442, rs719366, rs1005533, rs722098, rs2830795, and rs987640.*discriminating SNPs used in chimerism calculation.Abbreviations: NGS, next generation sequencing; SNPs, single nucleotide polymorphisms.

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