J Korean Gastric Cancer Assoc.  2007 Jun;7(2):59-66.

Multiple Genetic Marker Analysis wih Using Quantitative RT-PCR in Gastric Cancer

• Yoo MW
• Lee HJ
• Choi SM
• Yu J
• Hur K
• Kim YK
• Yang HK

Abstract

PURPOSE: This study was aimed at evaluating the diagnostic validity of peritoneal dissemination of gastric cancer cells by performing multiple genetic marker analysis via quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in gastric cancer cell lines and gastric cancer tissues.
MATERIALS AND METHODS
Quantitative RT-PCR was performed on 12 human gastric cancer cell lines and 10 gastric cancer tissues with four mRNAs of carcinoembryonic antigen (CEA), Cytokeratin 20 (CK20), dopa decarboxylase (DDC), and L-3-phosphoserine phosphatase (L3PP).
RESULTS
Out of the 12 human gastric cancer cell lines we tested, CEA was overexpressed in four cell lines (33%), CK20 in one (8%), DDC in six (50%) and L3PP was expessed in all the lines (100%). Out of the 10 gastric cancer tissues we tested, CEA was overexpressed in nine tissues, CK20 in eight, DDC in nine and L3PP was overexpressed in all the tissues. L3PP was overexpressed in all the gastric cancer cell lines and tissues, but the levels of overexpression were lower than those of CEA and DDC.
CONCLUSION
Multiple genetic marker analysis can compensate for the weak points of single marker analysis when testing gastric cancer, and three mRNAs of CEA, DDC and L3PP can be used as candidate genes.

Keyword

Multiple marker; RT-PCR; Gastric cancer

MeSH Terms

Carcinoembryonic Antigen
Cell Line
Dopa Decarboxylase
Genetic Markers*
Humans
Keratin-20
RNA, Messenger
Stomach Neoplasms*
Carcinoembryonic Antigen
Dopa Decarboxylase
Genetic Markers
Keratin-20
RNA, Messenger