Korean J Physiol Pharmacol.  2017 Jan;21(1):11-17. 10.4196/kjpp.2016.21.1.11.

Association study between OCTN1 functional haplotypes and Crohn's disease in a Korean population

• Jung ES
• Park HJ
• Kong KA
• Choi JH
• Cheon JH

• KMID: 2371063
• DOI: http://doi.org/10.4196/kjpp.2016.21.1.11

Abstract

Crohn's disease (CD) is a chronic inflammatory bowel disease with multifactorial causes including environmental and genetic factors. Several studies have demonstrated that the organic cation/carnitine transporter 1 (OCTN1) non-synonymous variant L503F is associated with susceptibility to CD. However, it was reported that L503F is absent in Asian populations. Previously, we identified and functionally characterized genetic variants of the OCTN1 promoter region in Koreans. In that study, four variants demonstrated significant changes in promoter activity. In the present study, we determined whether four functional variants of the OCTN1 promoter play a role in the susceptibility to or clinical course of CD in Koreans. To examine it, the frequencies of the four variants of the OCTN1 promoter were determined by genotyping using DNA samples from 194 patients with CD and 287 healthy controls. Then, associations between genetic variants and the susceptibility to CD or clinical course of CD were evaluated. We found that susceptibility to CD was not associated with OCTN1 functional promoter variants or haplotypes showing altered promoter activities in in vitro assays. However, OCTN1 functional promoter haplotypes showing decreased promoter activities were significantly associated with a penetrating behavior in CD patients (HR=2.428, p=0.009). Our results suggest that the OCTN1 functional promoter haplotypes can influence the CD phenotype, although these might not be associated with susceptibility to this disease.

Keyword

Association; Crohn's disease; Haplotype; OCTN1; Phenotype

MeSH Terms

Asian Continental Ancestry Group
Crohn Disease*
DNA
Haplotypes*
Humans
In Vitro Techniques
Inflammatory Bowel Diseases
Phenotype
Promoter Regions, Genetic
DNA

Figure

• 

  Fig. 1 Kaplan-Meier curve for developing a penetrating disease behavior in Korean CD patients during follow-up.The log-rank test was performed between the OCTN1 variant (solid line) and control (dashed line) groups (p=0.008).

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