Ann Dermatol.  2016 Dec;28(6):772-775. 10.5021/ad.2016.28.6.772.

Betamethasone Butyrate Propionate Inhibits the Induction of Thymic Stromal Lymphopoietin in Cultured Normal Human Keratinocytes

• Zhang W
• Sakai T
• Hatano Y
• Fujiwara S

• KMID: 2368133
• DOI: http://doi.org/10.5021/ad.2016.28.6.772

Abstract

No abstract available.

MeSH Terms

Betamethasone*
Butyrates*
Diethylpropion*
Humans*
Keratinocytes*
Betamethasone
Butyrates
Diethylpropion

Figure

• 

  Fig. 1 Effects of betamethasone butyrate propionate on the expression of thymic stromal lymphopoietin (TSLP) in cultured normal human keratinocytes (NHKs). Expressions of TSLP in cultured NHKs were induced by stimulation with poly I:C (10 µg/ml), tumor necrosis factor-α (20 ng/ml) and interleukin-4 (100 ng/ml) with or without the indicated dose (M) of betamethasone butyrate propionate (BBP). NHKs for real-time polymerase chain reaction (A) and cultured supernatants for ELISA (B) were harvested at 6 h and 24 h after stimulation, respectively. n=5 in (A) and (B). Error bars represent means±SEMs. p-values, vs. experimental group without BBP: *p<0.001, **p<0.0005, ***p<0.0001, ****p<0.00005, *****p<0.00001.

• 

  Fig. 2 Comparison of the effects on thymic stromal lymphopoietin (TSLP) expression in cultured normal human keratinocytes (NHKs) between betamethasone butyrate propionate (BBP) and dexamethasone (Dex) or prednisolone (PSL). Expressions of TSLP in cultured NHKs were induced by stimulation with poly I:C (10 µg/ml), tumor necrosis factor-α (20 ng/ml) and interleukin-4 (100 ng/ml) with or without indicated dose (M) of BBP, Dex (A, B), or PSL (C, D). NHKs for real-time polymerase chain reaction (A, C) and cultured supernatants for ELISA (B, D) were harvested at 6 h and 24 h after stimulation, respectively. n=5. Error bars represent means±SEMs. *p<0.005, **p<0.001, ***p<0.0005.

Reference

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