Ann Lab Med.  2015 May;35(3):366-369. 10.3343/alm.2015.35.3.366.

A Novel Case of Extreme Thrombocytosis in Acute Myeloid Leukemia Associated With Isochromosome 17q and Copy Neutral Loss of Heterozygosity

• You E
• Cho SY
• Yang JJ
• Lee HJ
• Lee WI
• Lee J
• Cho KS
• Cho EH
• Park TS

• KMID: 2363212
• DOI: http://doi.org/10.3343/alm.2015.35.3.366

Abstract

No abstract available.

MeSH Terms

Aged
Bone Marrow/pathology
Genome, Human
Humans
Isochromosomes/*genetics
Karyotyping
Leukemia, Myeloid, Acute/complications/*diagnosis/genetics
*Loss of Heterozygosity
Male
Oligonucleotide Array Sequence Analysis
Thrombocytosis/*etiology

Figure

• 

  Fig. 1 The findings of the peripheral blood (PB) smear and bone marrow (BM) aspiration. The PB smear shows leukemic blasts (horizontal arrow) (A). Dysplastic features were found on the PB smear such as marked anisopoikilocytosis in red blood cells (RBCs), pseudo-Pelger-Huet-like neutrophils (vertical arrow), and extreme thrombocytosis with giant and large platelets (B). The BM aspirate smear shows that hematopoietic components are barely visible owing to extreme thrombocytosis (C) and micromegakaryocytes display dyspoietic features of binucleation or non-lobulated shapes (D). Wright-Giemsa; ×200 (C), ×1,000 (A, B, D).

• 

  Fig. 2 Chromosome and microarray analyses. Giemsa-banded karyogram of the bone marrow cells at diagnosis: 46,XY,i(17)(q10). The arrow denotes the abnormal chromosome (A). Microarray analysis shows a single copy loss in chromosome 17 at bands p13.3 through p11.2, and a single copy gain of chromosome 17 at bands p11.2 through q25.3 [arr 17p13.3p11.2(64,214-18,751,820)×1, 17p11.2q25.3(18,751,820-80,587,411)×3] (B). SNP array analysis shows homozygosity in the long arm of chromosome 7, at band q11.1, which is approximately 135.9 megabases [arr 7q11.1q36(59,000,001-159,138,663)×2 homozygous (hmz)] (Affymetrix cytogenetics 2.7M array; Affymetrix, Santa Clara, CA, USA) (C).

Reference

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