Tissue Eng Regen Med.  2016 Jun;13(3):297-303. 10.1007/s13770-016-9054-9.

Local Injection of Granulocyte-Colony Stimulating Factor Accelerates Wound Healing in a Rat Excisional Wound Model

• Shen GY
• Park IH
• Song YS
• Joo HW
• Lee Y
• Shin JH
• Kim KS
• Kim H

• KMID: 2361014
• DOI: http://doi.org/10.1007/s13770-016-9054-9

Abstract

A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.

Keyword

Granulocyte-colony stimulating factor; Wound healing; Local injection

MeSH Terms

Animals
Blotting, Western
Collagen
Gene Expression
Granulocyte Colony-Stimulating Factor
Models, Animal
Rats*
Receptors, Granulocyte Colony-Stimulating Factor
Skin
Wound Healing*
Wounds and Injuries*
Collagen
Granulocyte Colony-Stimulating Factor
Receptors, Granulocyte Colony-Stimulating Factor