Korean J Parasitol.  2015 Dec;53(6):675-682. 10.3347/kjp.2015.53.6.675.

Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella

Affiliations
  • 1College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China. haustzbh@163.com
  • 2Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA.

Abstract

The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.

Keyword

Eimeria tenella; diclazuril; bursa of Fabricius (BF); secretory immunoglobulin A (SIgA); ultrastructure; chicken

MeSH Terms

Animals
Bursa of Fabricius/anatomy & histology/*parasitology
Chickens
Coccidiosis/drug therapy/metabolism/parasitology/*veterinary
Coccidiostats/administration & dosage/*adverse effects
Eimeria tenella/*physiology
Female
Immunoglobulin A, Secretory/*genetics/metabolism
Male
Nitriles/administration & dosage/*adverse effects
Poultry Diseases/*drug therapy/genetics/metabolism/parasitology
Triazines/administration & dosage/*adverse effects
Coccidiostats
Immunoglobulin A, Secretory
Nitriles
Triazines
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