Lab Anim Res.  2016 Sep;32(3):171-179. 10.5625/lar.2016.32.3.171.

Anti-atherosclerotic effects of perilla oil in rabbits fed a high-cholesterol diet

• Cha Y
• Jang JY
• Ban YH
• Guo H
• Shin K
• Kim TS
• Lee SP
• Choi J
• An ES
• Seo DW
• Yon JM
• Choi EK
• Kim YB

• KMID: 2353593
• DOI: http://doi.org/10.5625/lar.2016.32.3.171

Abstract

Anti-atherosclerosis effects of perilla oil were investigated, in comparison with lovastatin, in rabbits fed a high-cholesterol diet (HCD). Hypercholesterolemia was induced in rabbits by feeding the HCD containing 0.5% cholesterol and 1% corn oil, and perilla oil (0.1 or 0.3%) was added to the diet containing 0.5% cholesterol for 10 weeks. HCD greatly increased blood total cholesterol and low-density lipoproteins, and caused thick atheromatous plaques, covering 74% of the aortic wall. Hyper-cholesterolemia also induced lipid accumulation in the liver and kidneys, leading to lipid peroxidation. Perilla oil not only attenuated hypercholesterolemia and atheroma formation, but also reduced fat accumulation and lipid peroxidation in hepatic and renal tissues. The results indicate that perilla oil prevents atherosclerosis and fatty liver by controlling lipid metabolism, and that it could be the first choice oil to improve diet-induced metabolic syndrome.

Keyword

Hypercholesterolemia; atherosclerosis; fatty liver; lipid peroxidation; perilla oil

MeSH Terms

Atherosclerosis
Cholesterol
Corn Oil
Diet*
Fatty Liver
Hypercholesterolemia
Kidney
Lipid Metabolism
Lipid Peroxidation
Lipoproteins, LDL
Liver
Lovastatin
Perilla*
Plaque, Atherosclerotic
Rabbits*
Cholesterol
Corn Oil
Lipoproteins, LDL
Lovastatin

Figure

• 

  Figure 1 Change in body weights of hypercholesterolemic rabbits fed a high-cholesterol diet (HCD) containing perilla oil or lovastatin for 10 weeks. ○, Normal diet; ●, HCD alone; ▼, HCD+0.1% perilla oil; ■, HCD+0.3% perilla oil; ◆, HCD+ 0.002% lovastatin.

• 

  Figure 2 Effects of perilla oil and lovastatin on the blood total cholesterol (TC), low-density lipoproteins (LDL), high-density lipoproteins (HDL), and triglycerides (TG) of high-cholesterol diet (HCD)-fed rabbits (% of initial concentration). ○, Normal diet; ●, HCD alone; ▼, HCD+0.1% perilla oil; ■, HCD+0.3% perilla oil; ◆, HCD+0.002% lovastatin. *Significantly different from normal, P<0.05. #Significantly different from HCD alone, P<0.05.

• 

  Figure 3 Findings of atheroma of rabbits fed a high-cholesterol diet (HCD) containing perilla oil or lovastatin for 10 weeks. *Significantly different from normal diet (ND), P<0.05. #Significantly different from HCD alone, P<0.05.

• 

  Figure 4 Representative microscopic findings of the liver of rabbits fed a high-cholesterol diet (HCD) containing perilla oil or lovastatin for 10 weeks. ND, normal diet.

• 

  Figure 5 Representative microscopic findings of the kidney of rabbits fed a high-cholesterol diet (HCD) containing perilla oil or lovastatin for 10 weeks. ND, normal diet.

• 

  Figure 6 Effects of perilla oil (0.1 or 0.3%) and lovastatin (0.002%) on the lipid peroxidation of liver (A) and kidney (B) tissues of high-cholesterol diet (HCD)-fed rabbits. ND, normal diet.

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