Ewha Med J.  2003 Jun;26(2):133-136. 10.12771/emj.2003.26.2.133.

Study of M.Tuberculosis and Non-Tuberculous Mycobacterium in Subacute Necrotizing Lymphadenitis Using Multiplex PCR

• Cho MS
• Han WS

• KMID: 2352865
• DOI: http://doi.org/10.12771/emj.2003.26.2.133

Abstract

OBJECTIVES
Subacute necrotizing lymphadenitis is a self-limiting disorder that typically affects cervical lymph nodes of young women. Although autoimmune reaction and a viral etiology have been suggested, the definite cause has been uncertain. We evaluated the association of M. tuberculosis and non-tuberculous mycobacterium with this disorder.
MATERIALS AND METHODS
From 1994 to 2002, Clinicopathological findings of 38 cases were reviewed. Multiplex nested PCR was done using DNA extracted from paraffin-embedded tissue. Sequencing analysis was done to the cases showing positive band.
RESULTS
3 cases among 38(7.89%) showed amplified non-tuberculous mycobacterium DNA. Two cases are M. gordonae and one is M. fortuitum by sequencing analysis. All cases revealed no positive band for M. tuberculosis. Negative control cases showed no bands for both mycobacteriums.
CONCLUSION
Although 7.89% of cases showed amplified non-tuberculous mycobacterium DNA, more study is needed to confirm the association of non-tuberculous mycobacterium with subacute necrotizing lymphadenitis.

Keyword

Subacute necrotizing lymphadenitis; Tuberculosis; Nontuberculous mycobacterium; Multiplex PCR

MeSH Terms

DNA
Female
Gordonia Bacterium
Humans
Lymph Nodes
Lymphadenitis*
Multiplex Polymerase Chain Reaction*
Mycobacterium*
Nontuberculous Mycobacteria
Polymerase Chain Reaction
Tuberculosis
DNA