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Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR

Kumari P, Sinha S, Gahtori R, Quadiri A, Mahale P, Savargaonkar D, Pande V, Srivastava B, Singh H, Anvikar AR

Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic...
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Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen

Park SH, Hwang KA, Ahn JH, Nam JH

Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of...
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Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae

Kim SH, Bae IK, Kim NY, Song SA, Kim S, Jeong J, Shin JH

BACKGROUND: The isolation of carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly common. Continuous surveillance for these organisms is essential because their infections are closely related to outbreaks of illness and are...
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Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples

Shin JH, Lee SE, Kim TS, Ma DW, Cho SH, Chai JY, Shin EH

This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes...
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Differential Diagnosis of Human Sparganosis Using Multiplex PCR

Jeon HK, Kim KH, Sohn WM, Eom KS

Human sparganosis was diagnosed by morphological and genetic analyses in Korea. The complete mitochondrial genomes of Spirometra erinaceieuropaei and S. decipiens isolated in Korea have been recorded. Present study was...
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Multiplex PCR Based Epidemiological Study for the Causes of Acute Diarrheal Disease in Adults Living in Jeju Island

Lee K, Kim DS, Chong MS

BACKGROUND: We attempted to determine the characteristics of diarrheal pathogens according to species, seasonal variations, and patient age using multiplex PCR for the epidemiologic study of diarrheal disease in Jeju...
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Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera

Ranjbar R, Naghoni A, Afshar D, Nikkhahi F, Mohammadi M

Objectives Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract...
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Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea

Shin JH, Lee SE, Kim TS, Ma DW, Chai JY, Shin EH

This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s...
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Comparison of the Ability of Multiplex and Singleplex PCR to Detect Human Respiratory Viruses

Kim S, Eom KW, Cho CR, Um TH

BACKGROUND: The use of the multiplex polymerase chain reaction (PCR) technique for respiratory viruses has become popular in Korea owing to its convenience and sensitivity. However, concerns remain with regard...
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Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate

Dyab AK, Galal LA, Mahmoud AE, Mokhtar Y

Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for...
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Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea

Goo YK, Shin WS, Yang HW, Joo SY, Song SM, Ryu JS, Lee WM, Kong HH, Lee WK, Lee SE, Lee WJ, Chung DI, Hong Y

This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained...
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Xenomonitoring of Different Filarial Nematodes Using Single and Multiplex PCR in Mosquitoes from Assiut Governorate, Egypt

Dyab AK, Galal LA, Mahmoud AE, Mokhtar Y

Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed...
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The Evaluation of Recovery Rate of Neisseria gonorrhoeae in Two Bacterial Transport Swab Systems and Prevalence of Co-Infection after Delayed Transport

Koo H, Seo Y, Lee Y, Lee H, Yong D, Jeong SH, Lee K

BACKGROUND: Neisseria gonorrhoeae infection remains prevalent, and the emergence of antimicrobial resistance has made the treatment and control of gonorrhea more difficult. Therefore, it is important to compare isolation methods...
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Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea

Seo JH, Yang HW, Joo SY, Song SM, Lee YR, Ryu JS, Yoo ES, Lee WK, Kong HH, Lee SE, Lee WJ, Goo YK, Chung DI, Hong Y

Trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. Despite the availability of highly sensitive diagnostic tests, such as nucleic...
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Detection of Respiratory Viruses and Atypical Bacterial Pathogens in Infants with Acute Respiratory Infections Using Multiplex PCR

Yoo SJ, Han JU, Shin BM

Multiplex PCR of nasopharyngeal aspirates detected viruses and atypical bacteria in 75.3% (219/291) of infants with acute respiratory infections from July 2010 to June 2013. Frequent viruses were human rhinovirus...
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Recent Situation of Taeniasis in Mongolia (2002-2012)

Davaasuren A, Dorjsuren , Yanagida T, Sako Y, Nakaya K, Davaajav A, Agvaandaram G, Enkhbat T, Gonchigoo , Dulmaa N, Chuluunbaatar G, Ito A

  • KMID: 1792172
  • Korean J Parasitol.
  • 2014 Apr;52(2):211-214.
Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia...
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Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

Park SB, Kwon K, Cha IS, Jang HB, Nho SW, Fagutao FF, Kim YK, Yu JE, Jung TS

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay...
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Diagnostic Utility of a Clonality Test for Lymphoproliferative Diseases in Koreans Using the BIOMED-2 PCR Assay

Kim Y, Choi YD, Choi C, Nam JH

  • KMID: 2141007
  • Korean J Pathol.
  • 2013 Oct;47(5):458-465.
BACKGROUND: A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain...
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A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level

Kim Y, Choi Y, Jeon BY, Jin H, Cho SN, Lee H

PURPOSE: The Mycobacterium tuberculosis complex comprises M. tuberculosis, M. bovis, M. bovis bacillus Calmette-Guerin (BCG) and M. africanum, and causes tuberculosis in humans and animals. Identification of Mycobacterium spp. and...
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Molecular Approaches to Taenia asiatica

Jeon HK, Eom KS

Taenia solium, T. saginata, and T. asiatica are taeniid tapeworms that cause taeniasis in humans and cysticercosis in intermediate host animals. Taeniases remain an important public health concerns in the...
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