- Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR
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Kumari P, Sinha S, Gahtori R, Quadiri A, Mahale P, Savargaonkar D, Pande V, Srivastava B, Singh H, Anvikar AR
- KMID: 2533129
- Korean J Parasitol.
- 2022 Aug;60(4):295-299.
- doi: 10.3347/kjp.2022.60.4.295
Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic... -
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- Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen
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Park SH, Hwang KA, Ahn JH, Nam JH
- KMID: 2471837
- J Bacteriol Virol.
- 2020 Mar;50(1):44-54.
- doi: 10.4167/jbv.2020.50.1.044
Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of... -
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- Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae
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Kim SH, Bae IK, Kim NY, Song SA, Kim S, Jeong J, Shin JH
- KMID: 2441651
- Ann Clin Microbiol.
- 2019 Mar;22(1):9-13.
- doi: 10.5145/ACM.2019.22.1.9
BACKGROUND: The isolation of carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly common. Continuous surveillance for these organisms is essential because their infections are closely related to outbreaks of illness and are... -
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- Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples
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Shin JH, Lee SE, Kim TS, Ma DW, Cho SH, Chai JY, Shin EH
- KMID: 2426746
- Korean J Parasitol.
- 2018 Oct;56(5):419-427.
- doi: 10.3347/kjp.2018.56.5.419
This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes... -
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- Differential Diagnosis of Human Sparganosis Using Multiplex PCR
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Jeon HK, Kim KH, Sohn WM, Eom KS
- KMID: 2416362
- Korean J Parasitol.
- 2018 Jun;56(3):295-300.
- doi: 10.3347/kjp.2018.56.3.295
Human sparganosis was diagnosed by morphological and genetic analyses in Korea. The complete mitochondrial genomes of Spirometra erinaceieuropaei and S. decipiens isolated in Korea have been recorded. Present study was... -
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- Multiplex PCR Based Epidemiological Study for the Causes of Acute Diarrheal Disease in Adults Living in Jeju Island
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Lee K, Kim DS, Chong MS
- KMID: 2414118
- Ann Clin Microbiol.
- 2018 Mar;21(1):1-7.
- doi: 10.5145/ACM.2018.21.1.1
BACKGROUND: We attempted to determine the characteristics of diarrheal pathogens according to species, seasonal variations, and patient age using multiplex PCR for the epidemiologic study of diarrheal disease in Jeju... -
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Rapid Molecular Approach for Simultaneous Detection of
Salmonella spp.,Shigella spp., andVibrio cholera -
Ranjbar R, Naghoni A, Afshar D, Nikkhahi F, Mohammadi M
- KMID: 2518296
- Osong Public Health Res Perspect.
- 2016 Dec;7(6):373-377.
- doi: 10.1016/j.phrp.2016.10.002
Objectives Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract... -
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- Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea
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Shin JH, Lee SE, Kim TS, Ma DW, Chai JY, Shin EH
- KMID: 2360527
- Korean J Parasitol.
- 2016 Oct;54(5):631-636.
- doi: 10.3347/kjp.2016.54.5.631
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s... -
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- Comparison of the Ability of Multiplex and Singleplex PCR to Detect Human Respiratory Viruses
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Kim S, Eom KW, Cho CR, Um TH
- KMID: 2353426
- Lab Med Online.
- 2016 Oct;6(4):240-245.
- doi: 10.3343/lmo.2016.6.4.240
BACKGROUND: The use of the multiplex polymerase chain reaction (PCR) technique for respiratory viruses has become popular in Korea owing to its convenience and sensitivity. However, concerns remain with regard... -
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- Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate
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Dyab AK, Galal LA, Mahmoud AE, Mokhtar Y
- KMID: 2348267
- Korean J Parasitol.
- 2016 Jun;54(3):265-272.
- doi: 10.3347/kjp.2016.54.3.265
Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for... -
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- Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea
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Goo YK, Shin WS, Yang HW, Joo SY, Song SM, Ryu JS, Lee WM, Kong HH, Lee WK, Lee SE, Lee WJ, Chung DI, Hong Y
- KMID: 2374021
- Korean J Parasitol.
- 2016 Feb;54(1):75-80.
- doi: 10.3347/kjp.2016.54.1.75
This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained... -
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- Xenomonitoring of Different Filarial Nematodes Using Single and Multiplex PCR in Mosquitoes from Assiut Governorate, Egypt
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Dyab AK, Galal LA, Mahmoud AE, Mokhtar Y
- KMID: 2381799
- Korean J Parasitol.
- 2015 Feb;53(1):77-83.
- doi: 10.3347/kjp.2015.53.1.77
Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed... -
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- The Evaluation of Recovery Rate of Neisseria gonorrhoeae in Two Bacterial Transport Swab Systems and Prevalence of Co-Infection after Delayed Transport
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Koo H, Seo Y, Lee Y, Lee H, Yong D, Jeong SH, Lee K
- KMID: 1971105
- Ann Clin Microbiol.
- 2014 Dec;17(4):110-114.
- doi: 10.5145/ACM.2014.17.4.110
BACKGROUND: Neisseria gonorrhoeae infection remains prevalent, and the emergence of antimicrobial resistance has made the treatment and control of gonorrhea more difficult. Therefore, it is important to compare isolation methods... -
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- Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea
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Seo JH, Yang HW, Joo SY, Song SM, Lee YR, Ryu JS, Yoo ES, Lee WK, Kong HH, Lee SE, Lee WJ, Goo YK, Chung DI, Hong Y
- KMID: 1792224
- Korean J Parasitol.
- 2014 Oct;52(5):551-555.
- doi: 10.3347/kjp.2014.52.5.551
Trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. Despite the availability of highly sensitive diagnostic tests, such as nucleic... -
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- Detection of Respiratory Viruses and Atypical Bacterial Pathogens in Infants with Acute Respiratory Infections Using Multiplex PCR
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Yoo SJ, Han JU, Shin BM
- KMID: 1971100
- Ann Clin Microbiol.
- 2014 Sep;17(3):86-90.
- doi: 10.5145/ACM.2014.17.3.86
Multiplex PCR of nasopharyngeal aspirates detected viruses and atypical bacteria in 75.3% (219/291) of infants with acute respiratory infections from July 2010 to June 2013. Frequent viruses were human rhinovirus... -
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- Recent Situation of Taeniasis in Mongolia (2002-2012)
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Davaasuren A, Dorjsuren , Yanagida T, Sako Y, Nakaya K, Davaajav A, Agvaandaram G, Enkhbat T, Gonchigoo , Dulmaa N, Chuluunbaatar G, Ito A
- KMID: 1792172
- Korean J Parasitol.
- 2014 Apr;52(2):211-214.
Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia... -
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- Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)
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Park SB, Kwon K, Cha IS, Jang HB, Nho SW, Fagutao FF, Kim YK, Yu JE, Jung TS
- KMID: 1737623
- J Vet Sci.
- 2014 Mar;15(1):163-166.
- doi: 10.4142/jvs.2014.15.1.163
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay... -
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- Diagnostic Utility of a Clonality Test for Lymphoproliferative Diseases in Koreans Using the BIOMED-2 PCR Assay
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Kim Y, Choi YD, Choi C, Nam JH
- KMID: 2141007
- Korean J Pathol.
- 2013 Oct;47(5):458-465.
BACKGROUND: A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain... -
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- A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
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Kim Y, Choi Y, Jeon BY, Jin H, Cho SN, Lee H
- KMID: 1793171
- Yonsei Med J.
- 2013 Sep;54(5):1220-1226.
- doi: 10.3349/ymj.2013.54.5.1220
PURPOSE: The Mycobacterium tuberculosis complex comprises M. tuberculosis, M. bovis, M. bovis bacillus Calmette-Guerin (BCG) and M. africanum, and causes tuberculosis in humans and animals. Identification of Mycobacterium spp. and... -
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- Molecular Approaches to Taenia asiatica
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Jeon HK, Eom KS
- KMID: 1501803
- Korean J Parasitol.
- 2013 Feb;51(1):1-8.
- doi: 10.3347/kjp.2013.51.1.1
Taenia solium, T. saginata, and T. asiatica are taeniid tapeworms that cause taeniasis in humans and cysticercosis in intermediate host animals. Taeniases remain an important public health concerns in the... -
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