J Korean Ophthalmol Soc.  2014 Jan;55(1):110-118.

The Effect of RGP Lens and Reverse Geometry Lens on Redistribution of Corneal Epithelial Cell in Rabbit

• Jeong YJ
• Lee HB
• Park SP

Abstract

PURPOSE
To investigate the migration and redistribution of rabbit corneal epithelial cells when wearing reverse geometry lens (RGL) or rigid gas permeable lens (RGP).
METHODS
In 30 rabbits, the right eyes were fitted with either RGL or RGP and the left eyes were untreated to serve as controls. The rabbits were sacrificed at 1, 3, 7, 10 and 14 days after lens fitting. The central and peripheral corneal thicknesses were measured by microscope and the ratio of right to left corneal thickness was calculated to evaluate the characteristics of change over time. By using the molecular probe 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin), the samples were examined with light microscope to determine the migration and redistribution of epithelial cells in the rabbit cornea.
RESULTS
No consistent changes in the thickness of both central and peripheral corneal epithelium were found. The corneal epithelial cells of both eyes with RGL and RGP reacted positively to NBD phallacidin. The fluorescence was most increased at day 3 of sacrifice in RGL cases and at day 7 in RGP cases, and then decreased in both cases. The corneal epithelium of eyes with RGL exhibited marked increase in the intensity of fluorescence compared to the eyes with RGP.
CONCLUSIONS
The corneal epithelium with RGL showed the strongest intensity of NBD phallacidin fluorescence. This result suggests that wearing RGL may induce the migration and redistribution of corneal epithelial cells.

Keyword

Corneal epithelial cell; Redistribution; Reverse geometry lens; 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin)

MeSH Terms

Cornea
Epithelial Cells*
Epithelium, Corneal
Fluorescence
Molecular Probes
Rabbits
Molecular Probes

Figure

• 

  Figure 1. Hematoxylin and eosin (H&E) staining of the central cornea (A) and peripheral cornea (B, C) in reverse geometry lens-wearing rabbit at 10 days (A, B: ×100; C: ×200).

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  Figure 2. Thickness ratio of each measurement to control in RGL group and RGP group. (A) Central epithelium. (B) Central stroma. (C) Peripheral epithelium. (D) Peripheral stroma.

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  Figure 3. A positive NBD phallacidin fluorescence of the corneal epithelial cells in each group according to the day of sacrifice after lens fitting. The rabbit in group A was fitted with reverse geometry lens (RGL) and the rabbit in group B was fitted with rigid gas permeable lens (RGP). The photographs show marked increase of NBD phallacidin fluorescence in the corneal epithelial cells in the eyes fitted with RGL. The most increased fluorescence is observed in eyes with RGL at day 3 (arrows). A, B = group; Number = the day of sacrifice.

Reference

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